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The H9c2 cell line, derived from the embryonic rat heart, was treated with arginine vasopressin (AVP; 1 \u03bcM) to induce a cellular model of hypertrophy. Rates of glycolysis and oxidation of glucose and palmitate were measured in nonhypertrophied and hypertrophied H9c2 cells, and the effects of inhibition of AMPK were determined. AMPK activity was inhibited by 6-[4-(2-piperidin-1- yl-ethoxy)-phenyl]-3-pyridin-4-yl-pyrrazolo-[1,5-a]pyrimidine (compound C) or by adenovirus-mediated transfer of dominant negative AMPK. Compared with nonhypertrophied cells, glycolysis was accelerated and palmitate oxidation was reduced with no significant alteration in glucose oxidation in hypertrophied cells, a metabolic profile similar to that of intact hypertrophied hearts. Inhibition of AMPK resulted in the partial reduction of glycolysis in AVP-treated hypertrophied H9c2 cells. Acute exposure of H9c2 cells to AVP also activated AMPK and accelerated glycolysis. These elevated rates of glycolysis were not altered by AMPK inhibition but were blocked by agents that interfere with Ca<jats:sup>2+<\/jats:sup>signaling, including extracellular EGTA, dantrolene, and 2-aminoethoxydiphenyl borate. We conclude that the acceleration of glycolysis in AVP-treated hypertrophied heart muscle cells is partially dependent on AMPK, whereas the acute glycolytic effects of AVP are AMPK independent and at least partially Ca<jats:sup>2+<\/jats:sup>dependent.<\/jats:p>","DOI":"10.1152\/ajpheart.00396.2008","type":"journal-article","created":{"date-parts":[[2009,4,18]],"date-time":"2009-04-18T00:33:38Z","timestamp":1240014818000},"page":"H1822-H1832","source":"Crossref","is-referenced-by-count":18,"title":["AMP-activated protein kinase influences metabolic remodeling in H9c2 cells hypertrophied by arginine vasopressin"],"prefix":"10.1152","volume":"296","author":[{"given":"Ramesh","family":"Saeedi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Varun V.","family":"Saran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sherry S. 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