{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T09:46:55Z","timestamp":1768038415424,"version":"3.49.0"},"reference-count":61,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2018,2,1]]},"abstract":"<jats:p>The ability of cardiomyocytes to recover from a proton load was examined in the hearts of exercise-trained and sedentary control rats in CO<jats:sub>2<\/jats:sub>\/[Formula: see text]-free media. Acidosis was created by the NH<jats:sub>4<\/jats:sub>Cl prepulse technique, and intracellular pH (pH<jats:sub>i<\/jats:sub>) was determined using fluorescence microscopy on carboxy-SNARF-1 AM-loaded isolated cardiomyocytes. CO<jats:sub>2<\/jats:sub>-independent pH<jats:sub>i<\/jats:sub>buffering capacity (\u03b2<jats:sub>i<\/jats:sub>) was measured by incrementally reducing the extracellular NH<jats:sub>4<\/jats:sub>Cl concentration in steps of 50% from 20 to 1.25 mM. \u03b2<jats:sub>i<\/jats:sub>increased as pH<jats:sub>i<\/jats:sub>decreased in both exercise-trained and sedentary control groups. However, the magnitude of increase in \u03b2<jats:sub>i<\/jats:sub>as a function of pH<jats:sub>i<\/jats:sub>was found to be significantly ( P &lt; 0.001) greater in the exercise-trained group compared with the sedentary control group. The rate of pH<jats:sub>i<\/jats:sub>recovery from an imposed proton load was found to not be different between the exercise-trained and control groups. The Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup>exchanger-dependent H<jats:sup>+<\/jats:sup>extrusion rate during the recovery from an imposed proton load, however, was found to be significantly greater in the exercise-trained group compared with the control group. By increasing \u03b2<jats:sub>i<\/jats:sub>and subsequently the Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup>exchanger-dependent H<jats:sup>+<\/jats:sup>extrusion rate, exercise training may provide cardiomyocytes with the ability to better handle an intracellular excess of H<jats:sup>+<\/jats:sup>generated during hypoxia\/ischemic insults and may serve in a cardioprotective role. These data may be predictive of two positive outcomes: 1) increased exercise tolerance by the heart and 2) a protective mechanism that limits the degree of myocardial acidosis and subsequent damage that accompanies ischemia-reperfusion stress.<\/jats:p><jats:p>NEW &amp; NOTEWORTHY The enhanced ability to deal with acidosis conferred by exercise training is likely to improve exercise tolerance and outcomes in response to myocardial ischemia-reperfusion injury.<\/jats:p>","DOI":"10.1152\/ajpheart.00405.2017","type":"journal-article","created":{"date-parts":[[2017,12,6]],"date-time":"2017-12-06T14:54:02Z","timestamp":1512572042000},"page":"H285-H292","source":"Crossref","is-referenced-by-count":2,"title":["pH recovery from a proton load in rat cardiomyocytes: effects of chronic exercise"],"prefix":"10.1152","volume":"314","author":[{"given":"Vennetia R.","family":"Danes","sequence":"first","affiliation":[{"name":"Molecular Cardiac Physiology Group, Simon Fraser University, Burnaby, 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