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Moreover, the influence of lifestyle factors such as habitual exercise on endothelial cell (EC) senescence is unknown. We tested the hypothesis that EC senescence increases with sedentary, but not physically active, aging and is associated with vascular endothelial dysfunction. Protein expression (quantitative immunofluorescence) of p53, a transcription factor related to increased cellular senescence, and the cyclin-dependent kinase inhibitors p21 and p16 were 116%, 119%, and 128% greater (all P &lt; 0.05), respectively, in ECs obtained from antecubital veins of older sedentary (60\u2009\u00b1\u20091 yr, n = 12) versus young sedentary (22\u2009\u00b1\u20091 yr, n = 9) adults. These age-related differences were not present (all P &gt; 0.05) in venous ECs from older exercising adults (57\u2009\u00b1\u20091 yr, n = 13). Furthermore, venous EC protein levels of p53 ( r\u2009=\u2009\u22120.49, P = 0.003), p21 ( r\u2009=\u2009\u22120.38, P = 0.03), and p16 ( r\u2009=\u2009\u22120.58, P = 0.002) were inversely associated with vascular endothelial function (brachial artery flow-mediated dilation). Similarly, protein expression of p53 and p21 was 26% and 23% higher (both P &lt; 0.05), respectively, in ECs sampled from brachial arteries of healthy older sedentary (63\u2009\u00b1\u20091 yr, n = 18) versus young sedentary (25\u2009\u00b1\u20091 yr, n = 9) adults; age-related changes in arterial EC p53 and p21 expression were not observed ( P &gt; 0.05) in older habitually exercising adults (59\u2009\u00b1\u20091 yr, n = 14). These data indicate that EC senescence is associated with sedentary aging and is linked to endothelial dysfunction. Moreover, these data suggest that prevention of EC senescence may be one mechanism by which aerobic exercise protects against endothelial dysfunction with age. <\/jats:p><jats:p> NEW &amp; NOTEWORTHY Our study provides novel evidence in humans of increased endothelial cell senescence with sedentary aging, which is associated with impaired vascular endothelial function. Furthermore, our data suggest an absence of age-related increases in endothelial cell senescence in older exercising adults, which is linked with preserved vascular endothelial function. <\/jats:p><jats:p> Listen to this article's corresponding podcast at http:\/\/ajpheart.podbean.com\/e\/aging-exercise-and-endothelial-cell-senescence\/ . <\/jats:p>","DOI":"10.1152\/ajpheart.00416.2017","type":"journal-article","created":{"date-parts":[[2017,10,2]],"date-time":"2017-10-02T21:25:21Z","timestamp":1506979521000},"page":"H890-H895","source":"Crossref","is-referenced-by-count":223,"title":["Endothelial cell senescence with aging in healthy humans: prevention by habitual exercise and relation to vascular endothelial function"],"prefix":"10.1152","volume":"313","author":[{"given":"Matthew J.","family":"Rossman","sequence":"first","affiliation":[{"name":"Integrative Physiology, University of Colorado Boulder, Boulder, 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