{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:22:01Z","timestamp":1773418921571,"version":"3.50.1"},"reference-count":37,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:p>Ischemia-reperfusion (IR) injury is a major insult to postcapillary venules. We hypothesized that IR increases postcapillary venular hydraulic conductivity and that IR-mediated changes in hydraulic conductivity result from temporally and mechanistically separate processes. A microcannulation technique was used to determine hydraulic conductivity (L<jats:sub>p<\/jats:sub>) in rat mesenteric postcapillary venules serially throughout ischemia (45 min) and reperfusion (5 h) induced by superior mesenteric artery occlusion and release. Mesenteric IR resulted in a biphasic increase in L<jats:sub>p<\/jats:sub>. White blood cell (WBC) adhesion slowly increased with maximal adhesion corresponding to the second peak ( P &lt; 0.005). After IR, tissue was harvested for RT-PCR analysis of ICAM-1, E-selectin, and P-selectin mRNA. Intercellular adhesion molecule-1 (ICAM-1) mRNA in the gut showed the most significant upregulation. Quantitative real-time PCR revealed that ICAM-1 mRNA was upregulated 60-fold in the gut. An ICAM-1 antibody was therefore used to determine the effect of WBC adhesion on L<jats:sub>p<\/jats:sub>during IR. ICAM-1 inhibition attenuated L<jats:sub>p<\/jats:sub>during the first peak and completely blocked the second peak ( P &lt; 0.005). When rats were fed a tungsten diet to inhibit xanthine oxidase and then underwent IR, L<jats:sub>p<\/jats:sub>was dramatically attenuated during the first peak and mildly decreased the second peak ( P &lt; 0.005). Inhibition of xanthine oxidase by oxypurinol decreased L<jats:sub>p<\/jats:sub>during IR by over 60% ( P &lt; 0.002). Tempol, a superoxide dismutase mimetic, decreased L<jats:sub>p<\/jats:sub>during IR by over 30% ( P &lt; 0.01). We conclude that IR induces a biphasic increase in postcapillary hydraulic conductivity. Reactive oxygen species impact both the first transient peak and the sustained second peak. However, the second peak is also dependent on WBC-endothelial cell adhesion. These serial measurements of postcapillary hydraulic conductivity may lead the way for optimal timing of pharmaceutical therapies in IR injury.<\/jats:p>","DOI":"10.1152\/ajpheart.00419.2008","type":"journal-article","created":{"date-parts":[[2008,9,13]],"date-time":"2008-09-13T00:34:42Z","timestamp":1221266082000},"page":"H2164-H2171","source":"Crossref","is-referenced-by-count":18,"title":["Ischemia-reperfusion injury in rats affects hydraulic conductivity in two phases that are temporally and mechanistically separate"],"prefix":"10.1152","volume":"295","author":[{"given":"Gregory P.","family":"Victorino","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren\u00e9 M.","family":"Ramirez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terry J.","family":"Chong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Curran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javid","family":"Sadjadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1097\/01.TA.0000070166.29649.F3"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.1993.1041"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(200002)190:3<255::AID-PATH526>3.0.CO;2-6"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000250390.49380.94"},{"key":"R5","unstructured":"Christman JW, Blackwell TS, Juurlink BH.Redox regulation of nuclear factor kappa B: therapeutic potential for attenuating inflammatory responses.Brain Pathol (Zurich)10: 153\u2013162, 2000."},{"key":"R6","unstructured":"Curry FEHV, Sarelius IH.Measurement of Permeability, Pressure, and Flow.New York: Elsevier, 1983."},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2006.03.002"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-007-0969-4"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(87)91112-0"},{"key":"R10","unstructured":"Davies MGJT, Hagen PO.Endothelial Physiology.London: Blackwell Science, 1999."},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200006000-00008"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1016\/S0098-2997(01)00010-3"},{"key":"R13","unstructured":"Granger D.Physiology and pathophysiology of the microcirculation.Prog Cardiovasc Med3: 123\u2013140, 1998."},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1549-8719.1999.tb00099.x"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1988.255.6.H1269"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1989.257.5.G683"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(98)00094-X"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)02197-9"},{"key":"R19","unstructured":"Halliwell B, Gutteridge JM, Cross CE.Free radicals, antioxidants, and human disease: where are we now?J Lab Clin Med119: 598\u2013620, 1992."},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119751"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/59.4.539"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.81.6.922"},{"key":"R23","doi-asserted-by":"crossref","unstructured":"Johnson JL, Rajagopalan KV, Cohen HJ.Molecular basis of the biological function of molybdenum. Effect of tungsten on xanthine oxidase and sulfite oxidase in the rat.J Biol Chem249: 859\u2013866, 1974.","DOI":"10.1016\/S0021-9258(19)43008-1"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1159\/000051029"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.74.2.336"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1994.tb36724.x"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1997.273.6.G1189"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1097\/01.TA.0000133571.64393.D2"},{"key":"R29","unstructured":"Neary PRH.Ischaemia-reperfusion injury and the systemic inflammatory response syndrome. In:Ischemia-Reperfusion Injury, edited by Grace PA. London: Blackwell Science, 1999, p. 123\u2013136."},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1097\/00005373-199809000-00033"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.66.1.59"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1016\/0026-2862(91)90081-L"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1989.257.5.H1740"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1997.82.3.866"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.2002.6412"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6060(05)80085-6"},{"key":"R37","unstructured":"Zikria BABJ, Oz MO, Carlson RW.Reperfusion Injuries and Clinical Capillary Leak Syndrome.Armonk, NY: Futura Publishing, 1994, p. 443\u2013492."}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00419.2008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T23:14:33Z","timestamp":1684538073000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpheart.00419.2008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,11]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2008,11]]}},"alternative-id":["10.1152\/ajpheart.00419.2008"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00419.2008","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,11]]}}}