{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:48:42Z","timestamp":1790617722582,"version":"4.1.0"},"reference-count":51,"publisher":"American Physiological Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2014,6,1]]},"abstract":"<jats:p>Large conductance Ca<jats:sup>2+<\/jats:sup>-activated K<jats:sup>+<\/jats:sup>channels (BK<jats:sub>Ca<\/jats:sub>) contribute to negative feedback regulation of smooth muscle cell (SMC) tone. However, the effects of aging on BK<jats:sub>Ca<\/jats:sub>function are unclear. We tested the hypothesis that aging alters SMC BK<jats:sub>Ca<\/jats:sub>function in superior epigastric arteries (SEAs) by using perforated patch recording of enzymatically isolated SMCs from 3- to 4-mo-old male C57BL\/6 mice (Young) and 24- to 26-mo-old male C57BL\/6 mice (Old). SMC capacitance from Young (15.7 \u00b1 0.4 pF; n = 110) was less than Old (17.9 \u00b1 0.5 pF; n = 104) ( P &lt; 0.05). SMCs displayed spontaneous transient outward currents (STOCs) at membrane potentials more positive than \u221230 mV; depolarization increased STOC amplitude and frequency ( P &lt; 0.05; n = 19\u201324). STOC frequency in Young (2.2 \u00b1 0.6 Hz) was less than Old (4.2 \u00b1 0.7 Hz) at \u221210 mV ( P &lt; 0.05, n = 27\u201330), with no difference in amplitude (1.0 \u00b1 0.1 vs. 0.9 \u00b1 0.1 pA\/pF, respectively). At +30 mV, STOC amplitude in Young (3.2 \u00b1 0.3 pA\/pF) was less than Old (5.0 \u00b1 0.5 pA\/pF; P &lt; 0.05, n = 61\u201367) with no difference in frequency (3.9 \u00b1 0.4 vs. 3.2 \u00b1 0.3 Hz, respectively). BK<jats:sub>Ca<\/jats:sub>blockers (1 \u03bcM paxilline, 100 nM iberiotoxin, 1 mM tetraethylammonium) or a ryanodine receptor antagonist (100 \u03bcM tetracaine) inhibited STOCs (n \u2265 6; P &lt; 0.05 each). Western blots revealed increased expression of BK<jats:sub>Ca<\/jats:sub>\u03b1-subunit protein in Old. Pressure myography revealed no effect of age on SEA maximal diameter, myogenic tone, or paxilline-induced constriction ( n = 10\u201312; P &gt; 0.05). Enhanced functional expression of SMC BK<jats:sub>Ca<\/jats:sub>-dependent STOCs in Old may represent an adaptation of resistance arteries to maintain functional integrity.<\/jats:p>","DOI":"10.1152\/ajpheart.00492.2013","type":"journal-article","created":{"date-parts":[[2014,4,5]],"date-time":"2014-04-05T03:06:32Z","timestamp":1396667192000},"page":"H1512-H1524","source":"Crossref","is-referenced-by-count":16,"title":["Aging increases capacitance and spontaneous transient outward current amplitude of smooth muscle cells from murine superior epigastric arteries"],"prefix":"10.1152","volume":"306","author":[{"given":"Sebastien","family":"Hayoz","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Bradley","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Michigan State University, East Lansing, Michigan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika M.","family":"Boerman","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, Missouri; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahra","family":"Nourian","sequence":"additional","affiliation":[{"name":"Dalton Cardiovascular Research Center, Columbia, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven S.","family":"Segal","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, Missouri; and"},{"name":"Dalton Cardiovascular Research Center, Columbia, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William F.","family":"Jackson","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2009.051250"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.2010.02244.x"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.262592"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.113.301514"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1986.sp016333"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035273"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1126\/science.1373909"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/62.7.696"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1080\/10739680490425985"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.95.2.503"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2005.12.001"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.100.2.164"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.87.6.474"},{"key":"B14","doi-asserted-by":"crossref","first-page":"11083","DOI":"10.1016\/S0021-9258(19)38560-6","volume":"265","author":"Galvez A","year":"1990","journal-title":"J Biol Chem"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.01148.x"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1080\/10739680801982808"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092340406"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00124.2010"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.189811"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.35.1.173"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1998.274.1.H27"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1038\/bjp.2008.276"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.3109\/10739689709148316"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.6.C1755"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.09183FP"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.91245.2008"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1021\/bi00185a021"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-200106000-00025"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1991.260.3.H927"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2005.00671.x"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.88.2.210"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-3623(97)00034-7"},{"key":"B33","doi-asserted-by":"crossref","first-page":"2207","DOI":"10.1242\/jcs.115.10.2207","volume":"115","author":"McCarron JG","year":"2002","journal-title":"J Cell Sci"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00666.2001"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00773.2011"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5236.633"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.268.4.C799"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.068676"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2004.01.011"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019392"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1998.85.1.68"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00013.2011"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1992"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-201x.2000.00703.x"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-3623(02)00128-3"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2006.08.005"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1080\/10409230902882090"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.222083"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00728.2010"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2009.169920"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00492.2013","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T23:40:46Z","timestamp":1648770046000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpheart.00492.2013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,1]]},"references-count":51,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2014,6,1]]}},"alternative-id":["10.1152\/ajpheart.00492.2013"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00492.2013","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,1]]}}}