{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:09:27Z","timestamp":1758924567010},"reference-count":25,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:p>Characteristically, uterine myometrial cells (MCs) are proliferative, inducing angiogenesis within the female reproductive organ. We evaluated whether MCs implanted into myocardium could also induce angiogenesis and restore heart function after injury. MCs were isolated from the adult rat uterus and cultured for three studies: 1) Intracellular VEGF levels were measured in MCs cultured with progesterone (10<jats:sup>\u221211<\/jats:sup>, 10<jats:sup>\u22129<\/jats:sup>, and 10<jats:sup>\u22127<\/jats:sup>M) ( n = 6 tests per group). 2) Blood vessel density was evaluated 8 days after MCs (3 \u00d7 10<jats:sup>6<\/jats:sup>or 6 \u00d7 10<jats:sup>6<\/jats:sup>), smooth muscle cells (SMCs), or endothelial cells ( n = 6 rats per group) were injected with matrigel into the subcutaneous tissue of adult rats. 3) MCs, SMCs (5 \u00d7 10<jats:sup>6<\/jats:sup>\/rat), or media were injected into a transmural scar 3 wk after cryoinjury in rat hearts ( n = 12 rats per group), and heart function, blood vessel density, and myocardial scar size and thickness were evaluated 5 wk later. In study 1, cultured MCs expressed VEGF, with levels significantly ( P &lt; 0.05) upregulated by progesterone at an optimal dose of 10<jats:sup>\u221211<\/jats:sup>M. In study 2, MCs injected into the subcutaneous tissue with matrigel induced significantly more blood vessels, especially large-diameter vessels, than did SMCs or endothelial cells ( P &lt; 0.01 for all groups). This angiogenic effect was greatest ( P &lt; 0.01) at higher doses of MCs and was enhanced by progesterone (10<jats:sup>\u221211<\/jats:sup>M). In study 3, MCs implanted into the injured myocardium increased blood vessel density at the implant area, reduced scar size, and improved cardiac function relative to SMCs and media. Overall, MCs induced angiogenesis in vitro and in vivo, prevented cardiac remodeling, and improved heart functional recovery after cardiac injury.<\/jats:p>","DOI":"10.1152\/ajpheart.00494.2006","type":"journal-article","created":{"date-parts":[[2006,6,17]],"date-time":"2006-06-17T01:17:26Z","timestamp":1150507046000},"page":"H2057-H2066","source":"Crossref","is-referenced-by-count":13,"title":["Myometrial cells induce angiogenesis and salvage damaged myocardium"],"prefix":"10.1152","volume":"291","author":[{"given":"Ming-Li","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiji","family":"Matsubayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard D.","family":"Weisel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren-Ke","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000043246.74879.CD"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1124\/mol.54.1.105"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00687-1"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1038\/ncpcardio0431"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)08113-1"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0990451"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-128X(00)00113-6"},{"key":"R8","unstructured":"Horvath KA.Results of prospective randomized controlled trials of transmyocardial laser revascularization.Heart Surg Forum5: 33\u201339, 2002."},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1038\/415234a"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2003-030820"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.104.500256"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1067\/mtc.2001.117623"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2004.03.023"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1117"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1067\/mtc.2002.121044"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1998.0882"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4975(96)00389-X"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1210\/mend.15.11.0734"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.84.6.5886"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000125294.04612.81"},{"key":"R22","unstructured":"Shifren JL, Tseng JF, Zaloudek CJ, Ryan IP, Meng YG, Ferrara N, Jaffe RB, and Taylor RN.Ovarian steroid regulation of vascular endothelial growth factor in the human endometrium: implications for angiogenesis during the menstrual cycle and in the pathogenesis of endometriosis.J Clin Endocrinol Metab81: 3112\u20133118, 1996."},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/4.5.509"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.4.2185"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200522326"},{"key":"R26","doi-asserted-by":"crossref","unstructured":"Zhang L, Rees MC, and Bicknell R.The isolation and long-term culture of normal human endometrial epithelium and stroma. Expression of mRNAs for angiogenic polypeptides basally and on oestrogen and progesterone challenges.J Cell Sci108: 323\u2013331, 1995.","DOI":"10.1242\/jcs.108.1.323"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00494.2006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,28]],"date-time":"2021-07-28T13:32:24Z","timestamp":1627479144000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpheart.00494.2006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11]]},"references-count":25,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["10.1152\/ajpheart.00494.2006"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00494.2006","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11]]}}}