{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T18:04:29Z","timestamp":1718647469680},"reference-count":30,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2002,2,1]]},"abstract":"<jats:p>10.1152\/ajpheart 00546.2001.\u2014Vascular smooth muscle cells (VSMC) from spontaneously hypertensive rats (SHR) exhibit increased cell growth compared with normotensive Wistar-Kyoto rats (WKY). ANG II stimulates growth via G<jats:sub>q<\/jats:sub>-protein-coupled signaling that involves changes in cytosolic intracellular Ca<jats:sup>2+<\/jats:sup>concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) and activation of protein kinase C (PKC) and mitogen-activated protein kinases. This study examines the role of the proline-rich tyrosine kinase 2 (PYK2) in hypertensive VSMC. Basal PYK2 phosphorylation in SHR VSMC was increased compared with WKY (0.44 \u00b1 0.02 vs. 0.20 \u00b1 0.02-fold). ANG II-induced activation of PYK2 in SHR VSMC was of greater magnitude (2.2 \u00b1 0.2-fold in SHR; 1.4 \u00b1 0.1-fold in WKY) and occurred more rapidly (peak activation at 2 min in SHR vs. 5 min in WKY). This effect was blocked by pretreatment with the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>chelator 1,2-bis(2-aminophenoxy)ethane- N,N,N\u2032,N\u2032-tetraacetic acid or the PKC inhibitor chelerythrine. Basal and ANG II-stimulated c-Fos expression was increased in SHR versus WKY VSMC. PYK2 downregulation with antisense oligonucleotides blocked ANG II-induced c-Fos expression. Increased PYK2 activation may be altered signaling cascades that regulate cell growth in hypertensive VSMC.<\/jats:p>","DOI":"10.1152\/ajpheart.00546.2001","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:00:38Z","timestamp":1425412838000},"page":"H457-H465","source":"Crossref","is-referenced-by-count":17,"title":["Altered PYK2 phosphorylation by ANG II in hypertensive vascular smooth muscle"],"prefix":"10.1152","volume":"282","author":[{"given":"Petra","family":"Rocic","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama 35294; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tina M.","family":"Griffin","sequence":"additional","affiliation":[{"name":"Cardiovascular Institute, Stritch School of Medicine, Loyola University, Maywood, Illinois 60153"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chastity N.","family":"McRae","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama 35294; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela A.","family":"Lucchesi","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama 35294; and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"II142","DOI":"10.1161\/01.HYP.19.2_Suppl.II142","volume":"19","author":"Bendhack LM","year":"1992","journal-title":"Hypertens"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113964"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.3.1711"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1038\/383547a0"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(95)00088-7"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2000.1110"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.10.5225"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.87.8.710"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.34.4.924"},{"key":"B10","first-page":"83","volume":"40","author":"Kanayama Y","year":"1994","journal-title":"Osaka City Med J"},{"key":"B11","doi-asserted-by":"crossref","first-page":"6483","DOI":"10.1016\/S0021-9258(19)50453-7","volume":"267","author":"Kato H","year":"1992","journal-title":"J Biol Chem"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.7.4815"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(99)00207-1"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1038\/376737a0"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.10.6146"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.78.6.962"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.1995.tb02844.x"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199305000-00009"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1989.257.6.H1755"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.280.1.C90"},{"key":"B21","first-page":"A1008","volume":"13","author":"Rocic P","year":"1999","journal-title":"FASEB J"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M101684200"},{"key":"B23","first-page":"108","volume":"65","author":"Rosendorff C.","year":"1998","journal-title":"Mt. 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