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To study a specific subgroup of second-order neurons in medial NTS, we anterogradely labeled arterial baroreceptor afferents of the aortic depressor nerve (ADN) with lipophilic fluorescent tracer (i.e., ADN+) and measured synaptic responses to solitary tract (ST) activation recorded from dye-identified neurons in medial NTS in horizontal brain stem slices. Every ADN+ NTS neuron received constant-latency ST-evoked excitatory postsynaptic currents (EPSCs) (jitter &lt;192 \u03bcs, SD of latency). Stimulus-recruitment profiles showed single thresholds and no suprathreshold recruitment, findings consistent with EPSCs arising from a single, branched afferent axon. Frequency-dependent depression of ADN+ EPSCs averaged \u223c70% for five shocks at 50 Hz, but single-shock failure rates did not exceed 4%. Whether adjacent ADN\u2212 or those from unlabeled animals, other second-order NTS neurons (jitters &lt;200 \u03bcs) had ST transmission properties indistinguishable from ADN+. Capsaicin (CAP; 100 nM) blocked ST transmission in some neurons. CAP-sensitive ST-EPSCs were smaller and failed over five times more frequently than CAP-resistant responses, whether ADN+ or from unlabeled animals. Variance-mean analysis of ST-EPSCs suggested uniformly high probabilities for quantal glutamate release across second-order neurons. While amplitude differences may reflect different numbers of contacts, higher frequency-dependent failure rates in CAP-sensitive ST-EPSCs may arise from subtype-specific differences in afferent axon properties. Thus afferent transmission within medial NTS differed by axon class (e.g., CAP sensitive) but was indistinguishable by source of axon (e.g., baroreceptor vs. nonbaroreceptor).<\/jats:p>","DOI":"10.1152\/ajpheart.00568.2008","type":"journal-article","created":{"date-parts":[[2008,9,13]],"date-time":"2008-09-13T00:34:42Z","timestamp":1221266082000},"page":"H2032-H2042","source":"Crossref","is-referenced-by-count":43,"title":["Comparison of baroreceptive to other afferent synaptic transmission to the medial solitary tract nucleus"],"prefix":"10.1152","volume":"295","author":[{"given":"Michael C.","family":"Andresen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James H.","family":"Peters","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.12.022"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.22.10380"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903350203"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2004000400012"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.56.030194.000521"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1995.74.4.1518"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4177-04.2005"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3502-07.2007"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00038.2008"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.132365"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2044-06.2006"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5176-05.2006"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-05-01904.2000"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00505.2002"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00573.2005"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/0034-5687(95)00045-F"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1993.264.5.H1674"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1838(94)00191-L"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2280"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.076885"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(83)90482-2"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2003.09.019"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(99)01520-9"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1038\/nn1455"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199904260-00004"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1986.sp016035"},{"key":"R27","doi-asserted-by":"crossref","unstructured":"Davis JA.Mouse and rat anesthesia and analgesia.Curr Protoc NeurosciJan: Appendix 4B, 2008.","DOI":"10.1002\/0471142301.nsa04bs42"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1985.sp015616"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.1981.sp002582"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1982.sp014040"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2001.85.5.2213"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-18-08222.2002"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2004.02.007"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1998.275.2.H632"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1996.271.6.H2218"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1999.277.3.R748"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(83)90012-X"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2004.06.022"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00429.2004"},{"key":"R40","doi-asserted-by":"crossref","unstructured":"Godement P, Vanselow J, Thanos S, Bonhoeffer F.A study in developing visual systems with a new method of staining neurones and their processes in fixed tissue.Development101: 697\u2013713, 1987.","DOI":"10.1242\/dev.101.4.697"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1296.008"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1126\/science.3576212"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0753-04.2004"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(80)90553-3"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00252.2006"},{"key":"R46","unstructured":"Kubin L, Davies RO.Central pathways of pulmonary and airway vagal afferents. 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