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Neutrophils interact with the damaged endothelium and initiate an inflammatory cascade at the site of injury. We have generated a novel translational targeted cell therapy for acute vascular injury using adenoviral vectors to overexpress IL8RA\/B and green fluorescent protein (GFP) on the surface of endothelial cells (ECs) derived from human induced pluripotent stem cells (HiPS-IL8RA\/B-ECs). We hypothesize that HiPS-IL8RA\/B-ECs transfused intravenously into rats with balloon injury of the carotid artery will target to the injured site and compete with neutrophils, thus inhibiting inflammation and neointima formation. Young adult male Sprague-Dawley rats underwent balloon injury of the right carotid artery and received intravenous transfusion of saline vehicle, 1.5 \u00d7 10<jats:sup>6<\/jats:sup> HiPS-ECs, 1.5 \u00d7 10<jats:sup>6<\/jats:sup> HiPS-Null-ECs, or 1.5 \u00d7 10<jats:sup>6<\/jats:sup> HiPS-IL8RA\/B-ECs immediately after endoluminal injury. Tissue distribution of HiPS-IL8RA\/B-ECs was analyzed by a novel GFP DNA qPCR method. Cytokine and chemokine expression and leukocyte infiltration were measured in injured and uninjured arteries at 24 h postinjury by ELISA and immunohistochemistry, respectively. Neointimal, medial areas, and reendothelialization were measured 14 days postinjury. HiPS-IL8RA\/B-ECs homed to injured arteries, inhibited inflammatory mediator expression and inflammatory cell infiltration, accelerated reendothelialization, and attenuated neointima formation after endoluminal injury while control HiPS-ECs and HiPS-Null-ECs did not. HiPS-IL8RA\/B-ECs transfused into rats with endoluminal carotid artery injury target to the injured artery and provide a novel strategy to treat vascular injury. <\/jats:p>","DOI":"10.1152\/ajpheart.00587.2015","type":"journal-article","created":{"date-parts":[[2016,1,22]],"date-time":"2016-01-22T20:41:55Z","timestamp":1453495315000},"page":"H705-H715","source":"Crossref","is-referenced-by-count":13,"title":["Targeted delivery of human iPS-ECs overexpressing IL-8 receptors inhibits neointimal and inflammatory responses to vascular injury in the rat"],"prefix":"10.1152","volume":"310","author":[{"given":"Samantha","family":"Giordano","sequence":"first","affiliation":[{"name":"Vascular Biology and Hypertension Program, Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangmin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pulmonary, Critical Care, Sleep and Allergy, College of Medicine, University of Illinois at Chicago, Chicago, Illinois;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisy","family":"Xing","sequence":"additional","affiliation":[{"name":"Vascular Biology and Hypertension Program, Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fadi","family":"Hage","sequence":"additional","affiliation":[{"name":"Vascular Biology and Hypertension Program, Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama;"},{"name":"Division of Cardiology, Birmingham Veterans Affairs Medical Center, Birmingham, Alabama;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne","family":"Oparil","sequence":"additional","affiliation":[{"name":"Vascular Biology and Hypertension Program, Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John P.","family":"Cooke","sequence":"additional","affiliation":[{"name":"Houston Methodist Research Institute, Houston, Texas;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jieun","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine, Stanford University, Stanford, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karina H.","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Cardiovascular Institute, Stanford University, Stanford, California;"},{"name":"Veterans Affairs Palo Alto Health Care System, Palo Alto, California; 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