{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T15:14:52Z","timestamp":1784128492939,"version":"3.55.0"},"reference-count":65,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2015,1,15]]},"abstract":"<jats:p> We investigated the effects of acute pyridostigmine (PYR) treatment, an acetylcholinesterase inhibitor, on arterial pressure (AP), heart rate (HR), cardiac sympathovagal balance, and the incidence of arrhythmias during the first 4 h after myocardial infarction (MI) in anesthetized rats. Male Wistar rats were implanted with catheters into the femoral artery and vein for AP recordings and drug administration. Rats received the autonomic receptor blockers methyl-atropine (1 mg\/kg iv) and propranolol (2 mg\/kg iv) at intervals of 15 min, 1 h after saline ( n = 16) or PYR (0.25 mg\/kg iv, n = 18), to indirectly assess sympathovagal balance. Acute treatment with PYR increased cardiac vagal (86 \u00b1 7 vs. 44 \u00b1 5 beats\/min) and decreased sympathetic tone (\u221231 \u00b1 8 vs. \u221269 \u00b1 7 beats\/min). Different animals were implanted with ECG electrodes and catheters. A large MI was induced via left coronary artery ligation after basal recordings. Rats received PYR ( n = 14) or saline ( n = 14) 10\u201315 min after MI, and the recordings lasted up to 4 h. In part of the animals, hearts were removed for connexin43 quantification after all procedures. MI elicited a fall in AP (\u221245 \u00b1 5 mmHg), a progressive rise in HR (26 \u00b1 14 beats\/min), and an increase in corrected QT interval (33 \u00b1 13 ms). PYR elicited a prompt bradycardia (\u221250 \u00b1 14 beats\/min) that returned to basal levels over time, and it prevented the lengthening of the corrected QT interval. Treatment with PYR increased by \u223c20% the occurrence of rats free of arrhythmias after MI. MI markedly decreased connexin43 in left ventricles, and PYR treatment partially prevented this decrease. <\/jats:p>","DOI":"10.1152\/ajpheart.00591.2014","type":"journal-article","created":{"date-parts":[[2014,11,22]],"date-time":"2014-11-22T06:07:17Z","timestamp":1416636437000},"page":"H101-H107","source":"Crossref","is-referenced-by-count":21,"title":["Cholinergic stimulation with pyridostigmine protects myocardial infarcted rats against ischemic-induced arrhythmias and preserves connexin43 protein"],"prefix":"10.1152","volume":"308","author":[{"given":"Fernanda Machado","family":"Santos-Almeida","sequence":"first","affiliation":[{"name":"Department of Physiology, School of Medicine of Ribeir\u00e3o Preto, University of S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brazil; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrique","family":"Gir\u00e3o","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Imaging and Life Sciences, School 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