{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T04:25:38Z","timestamp":1774412738392,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","funder":[{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01 HL 146558"],"award-info":[{"award-number":["R01 HL 146558"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20 GM113125"],"award-info":[{"award-number":["P20 GM113125"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"abstract":"<jats:p> Changes in endothelial function precede the development of cardiovascular disease (CVD). We have previously shown that age-related declines in endothelial function in women are due in part to a reduction in endothelial cell endothelin-B receptor (ET<jats:sub>B<\/jats:sub>R) protein expression. However, it is not known if ET<jats:sub>B<\/jats:sub>R protein expression changes with aging in men. The purpose of this study was to test the hypothesis that ET<jats:sub>B<\/jats:sub>R protein expression is attenuated in older men (OM) compared to younger men (YM). Primary endothelial cells were harvested from the antecubital vein of 14 OM (60\u00b16 yrs; 26\u00b13 kg\/m<jats:sup>2<\/jats:sup>) and 17 YM (24\u00b15 yrs; 24\u00b12 kg\/m<jats:sup>2<\/jats:sup>). Cells were stained with 4',6-diamidino-2-phenylindole, vascular endothelial cadherin, and ET<jats:sub>B<\/jats:sub>R. Images were quantified using immunocytochemistry. Endothelial function was assessed using brachial artery flow-mediated dilation (FMD). Systolic BP was similar (OM: 123\u00b111 vs. YM: 122\u00b110 mmHg) whereas diastolic BP was higher in OM (OM: 77\u00b17 vs. YM: 70\u00b16 mmHg; P&lt;0.01). Total testosterone was lower in OM (OM: 6.28\u00b14.21 vs. YM: 9.10 \u00b12.68ng\/mL; P=0.03). As expected, FMD was lower in OM (OM: 3.85\u00b11.51 vs. YM: 6.40 \u00b12.68%; P&lt;0.01). However, ET<jats:sub>B<\/jats:sub>R protein expression was similar between OM and YM (OM: 0.39\u00b10.17 vs. YM: 0.42\u00b10.17 AU; P=0.66). These data suggest that ET<jats:sub>B<\/jats:sub>R protein expression is not altered with age in men. These findings are in contrast to our previous data in women, and further support sex differences in the endothelin system. <\/jats:p>","DOI":"10.1152\/ajpheart.00621.2023","type":"journal-article","created":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T14:56:47Z","timestamp":1699023407000},"update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Antecubital Venous Endothelial ETB Receptor Protein Expression is Preserved with Aging in Men"],"prefix":"10.1152","author":[{"given":"Saumya","family":"Tummala","sequence":"first","affiliation":[{"name":"University of Delaware, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew V.","family":"Kuczmarski","sequence":"additional","affiliation":[{"name":"Department of Kinesiology and Applied Physiology, University of Delaware, Newark, DE, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelica R.","family":"Del Vecchio","sequence":"additional","affiliation":[{"name":"Department of Kinesiology & Applied Physiology, University of Delaware, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allyson I.","family":"Schwab","sequence":"additional","affiliation":[{"name":"Department of Kinesiology & Applied Physiology, University of Delaware, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David G.","family":"Edwards","sequence":"additional","affiliation":[{"name":"Department of Kinesiology and Applied Physiology, University of Delaware, Newark, DE, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan M.","family":"Wenner","sequence":"additional","affiliation":[{"name":"Department of Kinesiology & Applied Physiology, University of Delaware, Newark, DE, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","published-online":{"date-parts":[[2023,11,3]]},"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00621.2023","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T14:56:49Z","timestamp":1699023409000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpheart.00621.2023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,3]]},"references-count":0,"alternative-id":["10.1152\/ajpheart.00621.2023"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00621.2023","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,3]]},"assertion":[{"value":"2023-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"ajpheart.00621.2023"}}