{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:31:52Z","timestamp":1792787512435,"version":"4.5.0"},"reference-count":75,"publisher":"American Physiological Society","issue":"1","funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["23PRE1021149"],"award-info":[{"award-number":["23PRE1021149"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01HD108839"],"award-info":[{"award-number":["R01HD108839"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["F31HL165818"],"award-info":[{"award-number":["F31HL165818"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Children\u2019s National Heart Institute"},{"DOI":"10.13039\/100011301","name":"Children\u2019s Research Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100011301","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"crossref","award":["24PRE1198328"],"award-info":[{"award-number":["24PRE1198328"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2024,7,1]]},"abstract":"<jats:p>\n                    We demonstrate that iCell cardiomyocytes\n                    <jats:sup>2<\/jats:sup>\n                    electrophysiology measurements are impacted by deviations in experimental techniques including electrical stimulation protocols, equilibration time, well-to-well variability, and length of hiPSC-CM culture. Furthermore, our results indicate that hiPSC-CM drug responsiveness changes within the first 2 wk following defrost.\n                  <\/jats:p>","DOI":"10.1152\/ajpheart.00631.2023","type":"journal-article","created":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T09:08:53Z","timestamp":1715332133000},"page":"H12-H27","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":22,"title":["hiPSC-CM electrophysiology: impact of temporal changes and study parameters on experimental reproducibility"],"prefix":"10.1152","volume":"327","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0025-5997","authenticated-orcid":false,"given":"Devon","family":"Guerrelli","sequence":"first","affiliation":[{"name":"Sheikh Zayed Institute for Pediatric Surgical Innovation, Children\u2019s National Hospital, Washington, District of Columbia, United States"},{"name":"Department of Biomedical Engineering, The George Washington University School of Engineering and Applied Science, Washington, District of Columbia, United States"},{"name":"Children\u2019s National Heart Institute, Children\u2019s National Hospital, Washington, District of Columbia, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4150-126X","authenticated-orcid":false,"given":"Jenna","family":"Pressman","sequence":"additional","affiliation":[{"name":"Sheikh Zayed Institute for Pediatric Surgical Innovation, Children\u2019s National Hospital, Washington, District of Columbia, United States"},{"name":"Department of Biomedical Engineering, The George Washington University School of Engineering and Applied Science, Washington, District of Columbia, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shatha","family":"Salameh","sequence":"additional","affiliation":[{"name":"Sheikh Zayed Institute for Pediatric Surgical Innovation, Children\u2019s National Hospital, Washington, District of Columbia, United States"},{"name":"Children\u2019s National Heart Institute, Children\u2019s National Hospital, Washington, District of Columbia, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3880-7200","authenticated-orcid":false,"given":"Nikki","family":"Posnack","sequence":"additional","affiliation":[{"name":"Sheikh Zayed Institute for Pediatric Surgical Innovation, Children\u2019s National Hospital, Washington, District of Columbia, United States"},{"name":"Children\u2019s National Heart Institute, Children\u2019s National Hospital, Washington, District of Columbia, United States"},{"name":"Department of Pediatrics, Department of Pharmacology and Physiology, The George Washington University School of Medicine and Health Sciences, Washington, District of Columbia, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1161\/HCG.0000000000000043"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1007\/S10557-017-6735-0"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1093\/EUROPACE\/EUAB142"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2015.34"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00439.2022"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.122.320258"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00694.2011"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.3389\/FPHYS.2018.00080\/BIBTEX"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.3389\/FPHYS.2012.00346"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.192237"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1007\/S004240000400\/METRICS"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.305365"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.113.001883"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-019-0331-x"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1242\/DEV.123810"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1016\/J.STEMCR.2019.08.013"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.311920"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2012.0490"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0016-3"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.307580"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCEP.113.003638"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2016.06.002"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1177\/1087057115594589"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfw200"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1093\/TOXSCI\/KFU312"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1002\/cptx.2"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2018.06.006"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-48174-5"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2017.12.003"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.08.079"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1093\/TOXSCI\/KFW235"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1093\/TOXSCI\/KFY110"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1254\/JPHS.13248FP"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2016.02.007"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2017.06.006"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1016\/J.BBAMCR.2015.12.013"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2015.04.002"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1152\/AJPHEART.00148.2020"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1152\/AJPHEART.00524.2023"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1093\/TOXSCI\/KFU186"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1111\/J.1476-5381.2011.01623.X"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.3791\/55587"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2022.107193"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1093\/TOXSCI\/KFT113"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1093\/TOXSCI\/KFU084"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1152\/AJPCELL.00306.2019"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1093\/TOXSCI\/KFZ235"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1093\/TOXSCI\/KFZ076"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1016\/J.VASCN.2016.12.003"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.3389\/FPHYS.2022.838435"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.120.017748"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0241362"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0167348"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfae015"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1016\/J.VASCN.2017.12.004"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-13590-y"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1007\/S00395-022-00973-0\/TABLES\/1"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-49653-5"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-25170-9"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2020.09.002"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1016\/J.JPHS.2019.06.006"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.15761\/tim.1000145"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfab083"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1093\/TOXSCI\/KFAA015"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1152\/AJPHEART.00283.2020"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1016\/J.VASCN.2015.12.002"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.119.315862"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1253\/CIRCJ.CJ-12-0987"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0040288"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2015.05.003"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.3390\/CELLS9102275"},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.1096\/FJ.15-280982\/-\/DC1"},{"key":"B74","doi-asserted-by":"publisher","DOI":"10.1016\/J.VASCN.2020.106915"},{"key":"B75","doi-asserted-by":"publisher","DOI":"10.3390\/CELLS10123370"},{"key":"B76","doi-asserted-by":"publisher","DOI":"10.1152\/AJPHEART.00819.2012"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00631.2023","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,9]],"date-time":"2024-06-09T14:17:09Z","timestamp":1717942629000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpheart.00631.2023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,1]]},"references-count":75,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,7,1]]}},"alternative-id":["10.1152\/ajpheart.00631.2023"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00631.2023","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.10.02.560475","asserted-by":"object"}]},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,1]]},"assertion":[{"value":"2023-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-30","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}