{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T11:39:41Z","timestamp":1790854781181,"version":"4.1.0"},"reference-count":61,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:p>The spontaneously hypertensive rat (SHR) is a model of cardiomyopathy characterized by a restricted use of exogenous long-chain fatty acid (LCFA) for energy production. The aims of the present study were to document the functional and metabolic response of the SHR heart under conditions of increased energy demand and the effects of a medium-chain fatty acid (MCFA; octanoate) supplementation in this situation. Hearts were perfused ex vivo in a working mode with physiological concentrations of substrates and hormones and subjected to an adrenergic stimulation (epinephrine, 10 \u03bcM).<jats:sup>13<\/jats:sup>C-labeled substrates were used to assess substrate selection for energy production. Compared with control Wistar rat hearts, SHR hearts showed an impaired response to the adrenergic stimulation as reflected by 1) a smaller increase in contractility and developed pressure, 2) a faster decline in the aortic flow, and 3) greater cardiac tissue damage (lactate dehydrogenase release: 1,577 \u00b1 118 vs. 825 \u00b1 44 mU\/min, P &lt; 0.01). At the metabolic level, SHR hearts presented 1) a reduced exogenous LCFA contribution to the citric acid cycle flux (16 \u00b1 1 vs. 44 \u00b1 4%, P &lt; 0.001) and an enhanced contribution of endogenous substrates (20 \u00b1 4 vs. 1 \u00b1 4%, P &lt; 0.01); and 2) an increased lactate production from glycolysis, with a greater lactate-to-pyruvate production ratio. Addition of 0.2 mM octanoate reduced lactate dehydrogenase release (1,145 \u00b1 155 vs. 1,890 \u00b1 89 mU\/min, P &lt; 0.001) and increased exogenous fatty acid contribution to energy metabolism (23.7 \u00b1 1.3 vs. 15.8 \u00b1 0.8%, P &lt; 0.01), which was accompanied by an equivalent decrease in unlabeled endogenous substrate contribution, possibly triglycerides (11.6 \u00b1 1.5 vs. 19.0 \u00b1 1.2%, P &lt; 0.01). Taken altogether, these results demonstrate that the SHR heart shows an impaired capacity to withstand an acute adrenergic stress, which can be improved by increasing the contribution of exogenous fatty acid oxidation to energy production by MCFA supplementation.<\/jats:p>","DOI":"10.1152\/ajpheart.00722.2004","type":"journal-article","created":{"date-parts":[[2004,11,19]],"date-time":"2004-11-19T01:24:14Z","timestamp":1100827454000},"page":"H1425-H1436","source":"Crossref","is-referenced-by-count":45,"title":["Fatty acid oxidation and its impact on response of spontaneously hypertensive rat hearts to an adrenergic stress: benefits of a medium-chain fatty acid"],"prefix":"10.1152","volume":"288","author":[{"given":"Fran\u00e7ois","family":"Labarthe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya","family":"Khairallah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertrand","family":"Bouchard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William C.","family":"Stanley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Des Rosiers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00638.2003"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9629(15)40570-1"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306285200"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1996.270.3.H817"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1991.261.3.H741"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.29.3.715"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5544"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1994.1155"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1994.267.5.H1862"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00287.2002"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.42.26117"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.42.26125"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000047668.48494.39"},{"key":"R14","doi-asserted-by":"crossref","unstructured":"De Vries JE, Vork MM, Roemen TH, de Jong YF, Cleutjens JP, van der Vusse GJ, and van Bilsen M.Saturated but not mono-unsaturated fatty acids induce apoptotic cell death in neonatal rat ventricular myocytes.J Lipid Res38: 1384\u20131394, 1997.","DOI":"10.1016\/S0022-2275(20)37421-6"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2002.2061"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4975(02)04692-1"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1998.274.4.H1239"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.45.29530"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100942200"},{"key":"R20","doi-asserted-by":"crossref","unstructured":"Horak ARand Opie LH.Energy metabolism of the heart in catecholamine-induced myocardial injury. Concentration-dependent effects of epinephrine on enzyme release, mechanical function, and \u201coxygen wastage\u201d.Adv Myocardiol4: 23\u201343, 1983.","DOI":"10.1007\/978-1-4757-4441-5_2"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1291\/hypres.26.829"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.2001.90.1.17"},{"key":"R23","unstructured":"Kohno I, Honma H, Nakamura T, and Tamura K.Comparison of blood pressure, heart rate and activity between normotensive and spontaneously-hypertensive rats.Chronobiologia21: 45\u201356, 1994."},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.278.4.H1345"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1994.1173"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2003.09.006"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000086964.07404.A5"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1995.268.6.H2505"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(98)00300-9"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1997.273.3.H1440"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.96.2.614"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1006\/phrs.1999.0652"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000136025.96811.76"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1982.tb07017.x"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01113.2003"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.279.5.H2390"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1016\/S1071-9164(98)90252-4"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.279.1.H26"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00244.2002"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4274(88)90081-1"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00387.2002"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M400162200"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012793924469"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215311"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1995.269.3.R630"},{"key":"R46","doi-asserted-by":"crossref","unstructured":"Saddik Mand Lopaschuk GD.Myocardial triglyceride turnover and contribution to energy substrate utilization in isolated working rat hearts.J Biol Chem266: 8162\u20138170, 1991.","DOI":"10.1016\/S0021-9258(18)92956-X"},{"key":"R47","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015380609464"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005556207210"},{"key":"R49","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1996.0128"},{"key":"R50","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.84.4.424"},{"key":"R51","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015320423577"},{"key":"R52","doi-asserted-by":"publisher","DOI":"10.1042\/bj3280913"},{"key":"R53","doi-asserted-by":"publisher","DOI":"10.1291\/hypres.26.485"},{"key":"R54","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2003.11.014"},{"key":"R55","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00717.2003"},{"key":"R56","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.2000.278.5.E846"},{"key":"R57","doi-asserted-by":"publisher","DOI":"10.1023\/A:1021189728877"},{"key":"R58","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.13.5.480"},{"key":"R59","doi-asserted-by":"publisher","DOI":"10.1161\/hy1101.096115"},{"key":"R60","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M103826200"},{"key":"R61","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)00664-1"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00722.2004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T02:37:43Z","timestamp":1624934263000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpheart.00722.2004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,3]]},"references-count":61,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,3]]}},"alternative-id":["10.1152\/ajpheart.00722.2004"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00722.2004","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,3]]}}}