{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T20:46:59Z","timestamp":1788468419608,"version":"build-2803163510"},"reference-count":34,"publisher":"American Physiological Society","issue":"8","funder":[{"DOI":"10.13039\/501100001809","name":"national science foundation of china","doi-asserted-by":"crossref","award":["31171096"],"award-info":[{"award-number":["31171096"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"national science foundation of china","doi-asserted-by":"crossref","award":["31371159"],"award-info":[{"award-number":["31371159"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"national science foundation of china","doi-asserted-by":"crossref","award":["81200122 to"],"award-info":[{"award-number":["81200122 to"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:p> In heterologous expression systems, KCNE2 has been demonstrated to interact with multiple \u03b1-subunits of voltage-dependent cation channels and modulate their functions. However, the physiological and pathological roles of KCNE2 in cardiomyocytes are poorly understood. The present study aimed to investigate the effects of bidirectional modulation of KCNE2 expression on action potential (AP) duration (APD) and voltage-dependent K<jats:sup>+<\/jats:sup> channels in cardiomyocytes. Adenoviral gene delivery and RNA interference were used to either increase or decrease KCNE2 expression in cultured neonatal and adult rat or neonatal mouse ventricular myocytes. Knockdown of KCNE2 prolonged APD in both neonatal and adult myocytes, whereas overexpression of KCNE2 shortened APD in neonatal but not adult myocytes. Consistent with the alterations in APD, KCNE2 knockdown decreased transient outward K<jats:sup>+<\/jats:sup> current ( I<jats:sub>to<\/jats:sub>) densities in neonatal and adult myocytes, whereas KCNE2 overexpression increased I<jats:sub>to<\/jats:sub> densities in neonatal but not adult myocytes. Furthermore, KCNE2 knockdown accelerated the rates of I<jats:sub>to<\/jats:sub> activation and inactivation, whereas KCNE2 overexpression slowed I<jats:sub>to<\/jats:sub> gating kinetics in neonatal but not adult myocytes. Delayed rectifier K<jats:sup>+<\/jats:sup> current densities were remarkably affected by manipulation of KCNE2 expression in mouse but not rat cardiomyocytes. Simulation of the AP of a rat ventricular myocyte with a mathematical model showed that alterations in I<jats:sub>to<\/jats:sub> densities and gating properties can result in similar APD alterations in KCNE2 overexpression and knockdown cells. In conclusion, endogenous KCNE2 in cardiomyocytes is important in maintaining cardiac electrical stability mainly by regulating I<jats:sub>to<\/jats:sub> and APD. Perturbation of KCNE2 expression may predispose the heart to ventricular arrhythmia by prolonging APD. <\/jats:p>","DOI":"10.1152\/ajpheart.00757.2014","type":"journal-article","created":{"date-parts":[[2015,8,22]],"date-time":"2015-08-22T01:30:59Z","timestamp":1440207059000},"page":"H1288-H1302","source":"Crossref","is-referenced-by-count":10,"title":["Manipulation of KCNE2 expression modulates action potential duration and <i>I<\/i><sub>to<\/sub> and <i>I<\/i><sub>K<\/sub> in rat and mouse ventricular myocytes"],"prefix":"10.1152","volume":"309","author":[{"given":"Wen-juan","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Pathophysiology, School of Medicine, Shenzhen University, Shenzhen, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-xin","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, The First Affiliated Hospital of Shenzhen University, Shenzhen No. 2 People's Hospital, Shenzhen, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, School of Medicine, Shenzhen University, Shenzhen, China; 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