{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T17:02:46Z","timestamp":1792602166423,"version":"4.5.0"},"reference-count":43,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2003,4,1]]},"abstract":"<jats:p>\n                    Rapid, nongenomic effects of 17\u03b2-estradiol (E\n                    <jats:sub>2<\/jats:sub>\n                    ) in endothelial cells are postulated to arise from membrane-associated estrogen receptors (ERs), which have not been visualized in vascular tissue. To identify membrane ERs, we used multiple site-directed ER\u03b1 or ER\u03b2 antibodies to label en face rat cerebral and coronary arterial endothelia. Western blots revealed a novel 55-kDa ER\u03b1 isoform. Three-dimensional images of cells labeled with these antibodies and markers for the nucleus and caveolin-1 were acquired with a wide-field microscope, deconvolved, and numerically analyzed. We found ER\u03b1 in the nucleus and cell periphery, where one-third colocalized with caveolin-1. The receptor location was dependent on the epitope of the antibody. Human ovarian surface epithelium produced similar results; but in rat myometrium, the distribution was epitope independent and nuclear. ER\u03b2 distribution was predominately intranuclear and epitope independent. A small amount of ER\u03b1 colocalized with ER\u03b2 within the nucleus. The results were identical in both arterial preparations and insensitive to E\n                    <jats:sub>2<\/jats:sub>\n                    . We postulate that the different ER\u03b1 conformations at the membrane, in the nucleus, and between different cell types allow E\n                    <jats:sub>2<\/jats:sub>\n                    to trigger cell- and location-specific signaling cascades.\n                  <\/jats:p>","DOI":"10.1152\/ajpheart.00781.2002","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T15:08:56Z","timestamp":1425395336000},"page":"H1295-H1306","source":"Crossref","is-referenced-by-count":62,"title":["Epitope-dependent localization of estrogen receptor\u03b1, but not -\u03b2, in en face arterial endothelium"],"prefix":"10.1152","volume":"284","author":[{"given":"Pauline","family":"Dan","sequence":"first","affiliation":[{"name":"Department of Physiology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joyce C. 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