{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T06:35:03Z","timestamp":1770273303924,"version":"3.49.0"},"reference-count":31,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:p>Thiamine is an important cofactor of metabolic enzymes, and its deficiency leads to cardiovascular dysfunction. First, we characterized the metabolic status measuring resting oxygen consumption rate and lactate blood concentration after 35 days of thiamine deficiency (TD). The results pointed to a decrease in resting oxygen consumption and a twofold increase in blood lactate. Confocal microscopy showed that intracellular superoxide (\u223c40%) and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>(2.5 times) contents had been increased. In addition, biochemical activities and protein expression of SOD, glutathione peroxidase, and catalase were evaluated in hearts isolated from rats submitted to thiamine deprivation. No difference in SOD activity was detected, but protein levels were found to be increased. Catalase activity increased 2.1 times in TD hearts. The observed gain in activity was attended by an increased catalase protein level. However, a marked decrease in glutathione peroxidase activity (control 435.3 \u00b1 28.6 vs. TD 199.4 \u00b1 30.2 nmol NADPH\u00b7min<jats:sup>\u22121<\/jats:sup>\u00b7ml<jats:sup>\u22121<\/jats:sup>) was paralleled by a diminution in the protein levels. Compared with control hearts, we did observe a greater proportion of apoptotic myocytes by TdT-mediated dUTP nick end labeling (TUNEL) and caspase-3 reactivity techniques. These results indicate that during TD, reactive oxygen species (ROS) production may be enhanced as a consequence of the installed acidosis. The perturbation in the cardiac myocytes redox balance was responsible for the increase in apoptosis.<\/jats:p>","DOI":"10.1152\/ajpheart.00820.2009","type":"journal-article","created":{"date-parts":[[2010,3,20]],"date-time":"2010-03-20T02:39:23Z","timestamp":1269052763000},"page":"H2039-H2045","source":"Crossref","is-referenced-by-count":49,"title":["Cardiac oxidative stress is involved in heart failure induced by thiamine deprivation in rats"],"prefix":"10.1152","volume":"298","author":[{"given":"Carolina Rosa","family":"Gioda","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Immunology,"},{"name":"Graduate Program in Physiology and Pharmacology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatiane","family":"de Oliveira Barreto","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thales Nicolau","family":"Pr\u00edmola-Gomes","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology,"},{"name":"Graduate Program in Physiology and Pharmacology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel Carvalho","family":"de Lima","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics,"},{"name":"Graduate Program in Physiology and Pharmacology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula Peixoto","family":"Campos","sequence":"additional","affiliation":[{"name":"Graduate Program in Physiology and Pharmacology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciano dos Santos Aggunn","family":"Capettini","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics,"},{"name":"Graduate Program in Physiology and Pharmacology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Lauton-Santos","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anilton C\u00e9sar","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Department of Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e2ndido C.","family":"Coimbra","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics,"},{"name":"Graduate Program in Physiology and Pharmacology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia Soares","family":"Lemos","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics,"},{"name":"Graduate Program in Physiology and Pharmacology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge L.","family":"Pesquero","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics,"},{"name":"Graduate Program in Physiology and Pharmacology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jader S.","family":"Cruz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology,"},{"name":"Graduate Program in Physiology and Pharmacology, and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2003.11.013"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00019.2006"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1079\/NRR200367"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2828(90)90073-B"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2006.10.048"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2007.06.729"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.101.1.33"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4927(03)00087-8"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1093\/eurjhf\/hfp146"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1974.36.3.391"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2005.04.025"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(90)90131-2"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1989.256.2.H368"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2007.06.033"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/j.npep.2007.01.002"},{"key":"B16","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","volume":"193","author":"Lowry OH","year":"1951","journal-title":"J Biol Chem"},{"key":"B17","volume-title":"Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology","author":"Luna LG","year":"1968"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)01054-X"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/100.8.991"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/S0047-6374(97)01876-9"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1177\/0148607194018002104"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(72)90451-4"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(79)90738-3"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.11.064"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00833.2006"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2005.02.032"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2009.03.011"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2003.07.047"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-483X(03)00151-3"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2005.12.009"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.055095"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00820.2009","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T01:16:32Z","timestamp":1635124592000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpheart.00820.2009"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6]]},"references-count":31,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,6]]}},"alternative-id":["10.1152\/ajpheart.00820.2009"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00820.2009","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,6]]}}}