{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T22:13:17Z","timestamp":1770070397669,"version":"3.49.0"},"reference-count":42,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:p>Extensive work has been done regarding the impact of thiamine deprivation on the nervous system. In cardiac tissue, chronic thiamine deficiency is described to cause changes in the myocardium that can be associated with arrhythmias. However, compared with the brain, very little is known about the effects of thiamine deficiency on the heart. Thus this study was undertaken to explore whether thiamine deprivation has a role in cardiac arrhythmogenesis. We examined hearts isolated from thiamine-deprived and control rats. We measured heart rate, diastolic and systolic tension, and contraction and relaxation rates. Whole cell voltage clamp was performed in rat isolated cardiac myocytes to measure L-type Ca<jats:sup>2+<\/jats:sup>current. In addition, we investigated the global intracellular calcium transients by using confocal microscopy in the line-scan mode. The hearts from thiamine-deficient rats did not degenerate into ventricular fibrillation during 30 min of reperfusion after 15 min of coronary occlusion. The antiarrhythmogenic effects were characterized by the arrhythmia severity index. Our results suggest that hearts from thiamine-deficient rats did not experience irreversible arrhythmias. There was no change in L-type Ca<jats:sup>2+<\/jats:sup>current density. Inactivation kinetics of this current in Ca<jats:sup>2+<\/jats:sup>-buffered cells was retarded in thiamine-deficient cardiac myocytes. The global Ca<jats:sup>2+<\/jats:sup>release was significantly reduced in thiamine-deficient cardiac myocytes. The amplitude of caffeine-releasable Ca<jats:sup>2+<\/jats:sup>was lower in thiamine-deficient myocytes. In summary, we have found that thiamine deprivation attenuates the incidence and severity of postischemic arrhythmias, possibly through a mechanism involving a decrease in global Ca<jats:sup>2+<\/jats:sup>release.<\/jats:p>","DOI":"10.1152\/ajpheart.00833.2006","type":"journal-article","created":{"date-parts":[[2007,3,17]],"date-time":"2007-03-17T01:31:05Z","timestamp":1174095065000},"page":"H394-H401","source":"Crossref","is-referenced-by-count":25,"title":["Abolition of reperfusion-induced arrhythmias in hearts from thiamine-deficient rats"],"prefix":"10.1152","volume":"293","author":[{"given":"Fernando A.","family":"Oliveira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Guatimosim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos H.","family":"Castro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diogo T.","family":"Galan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Lauton-Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela M.","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alvair P.","family":"Almeida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jader S.","family":"Cruz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Bernauer W, Ernenputsch I.Antagonistic effects of alpha-adrenoceptor blocking agents on arrhythmias, enzyme release, and myocardial necrosis in isolated rat hearts with coronary occlusion and reperfusion.Naunyn Schmiedebergs Arch Pharmacol338: 88\u201395, 1988.","DOI":"10.1007\/BF00168817"},{"key":"R2","doi-asserted-by":"crossref","unstructured":"Bers DM.Excitation-Contration Coupling and Cardiac Contractile Force. Dordrecht, The Netherlands: Kluwer Academic, 2002.","DOI":"10.1007\/978-94-010-0658-3"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.025239"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1989.tb14637.x"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2828(90)90073-B"},{"key":"R6","doi-asserted-by":"crossref","unstructured":"Cohen EM, Abelmann WH, Messer JV, Bing HL.Mechanical properties of rat cardiac muscle during experimental thiamine deficiency.Am J Physiol231: 1390\u20131394, 1976.","DOI":"10.1152\/ajplegacy.1976.231.5.1390"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/0922-4106(94)90142-2"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711020204"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.38.3.665"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5313.800"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117504"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000126294.81407.7D"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050423"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1998.275.3.H977"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.00795.x"},{"key":"R16","doi-asserted-by":"crossref","unstructured":"Jennings RB, Reimer KA.Pathobiology of acute myocardial ischemia.Hosp Pract (Off Ed)24: 89\u2013107, 1989.","DOI":"10.1080\/21548331.1989.11703644"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1996.0146"},{"key":"R18","unstructured":"Larrieu AJ, Yazdanfar S, Redovan E, Eftychiadis A, Kao R, Silver J, Ghosh SC.Beneficial effects of cocarboxylase in the treatment of experimental myocardial infarction in dogs.Am Surg53: 721\u2013725, 1987."},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.97.24.2463"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2003.12.031"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/12.4.212"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1046\/j.1525-1373.2000.22319.x"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(94)90109-0"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1990.tb15426.x"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(98)00300-9"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2003.10.012"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.11.064"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1016\/S0899-9007(00)00588-8"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-3057(01)00593-7"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01153.2004"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.021bi.x"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(99)80349-0"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.2174\/1566524013363870"},{"key":"R34","unstructured":"Stryer L, Tymoczko JL, Berg JM.Biochemistry.New York: W. H. Freeman, 2004."},{"key":"R35","doi-asserted-by":"crossref","unstructured":"Suter PM, Vetter W.Diuretics and vitamin B1: are diuretics a risk factor for thiamin malnutrition?Nutr Rev58: 319\u2013323, 2000.","DOI":"10.1111\/j.1753-4887.2000.tb01827.x"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.055095"},{"key":"R37","unstructured":"Vinogradov VV, Shneider AB, Senkevich SB.Thiamine cardiotropism.Cor Vasa33: 254\u2013262, 1991."},{"key":"R38","doi-asserted-by":"crossref","unstructured":"Warnock LG.Transketolase activity of blood hemolysate, a useful index for diagnosing thiamine deficiency.Clin Chem21: 432\u2013436, 1975.","DOI":"10.1093\/clinchem\/21.3.432"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(97)00178-0"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5223(95)70062-5"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.040345"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(95)00003-A"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00833.2006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T22:25:46Z","timestamp":1587421546000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpheart.00833.2006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,7]]}},"alternative-id":["10.1152\/ajpheart.00833.2006"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00833.2006","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,7]]}}}