{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:07:37Z","timestamp":1758924457071},"reference-count":29,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:p> The potassium channel blocker tetraethylammonium blocks the flow-induced increase in endothelial ICAM-1. We have investigated the subtype of potassium channel that modulates flow-induced increased expression of ICAM-1 on saphenous vein endothelium. Cultured human saphenous vein endothelial cells (HSVECs) or intact saphenous veins were perfused at fixed low and high flows in a laminar shear chamber or flow rig, respectively, in the presence or absence of potassium channel blockers. Expression of K<jats:sup>+<\/jats:sup> channels and endothelial ICAM-1 was measured by real-time polymerase chain reaction and\/or immunoassays. In HSVECs, the application of 0.8 N\/m<jats:sup>2<\/jats:sup> (8 dyn\/cm<jats:sup>2<\/jats:sup>) shear stress resulted in a two- to fourfold increase in cellular ICAM-1 within 6 h ( P &lt; 0.001). In intact vein a similar shear stress, with pulsatile arterial pressure, resulted in a twofold increase in endothelial ICAM-1\/CD31 staining area within 1.5 h ( P &lt; 0.001). Both increases in ICAM-1 were blocked by inclusion of 100 nM apamin in the vein perfusate, whereas other K<jats:sup>+<\/jats:sup> channel blockers were less effective. Two subtypes of small conductance Ca<jats:sup>2+<\/jats:sup>-activated K<jats:sup>+<\/jats:sup> channel (selectively blocked by apamin) were expressed in HSVECs and vein endothelium (SK3&gt;SK2). Apamin blocked the upregulation of ICAM-1 on saphenous vein endothelium in response to increased flow to implicate small conductance Ca<jats:sup>2+<\/jats:sup>-activated K<jats:sup>+<\/jats:sup> channels in shear stress\/flow-mediated signaling pathways. <\/jats:p>","DOI":"10.1152\/ajpheart.00880.2003","type":"journal-article","created":{"date-parts":[[2004,2,16]],"date-time":"2004-02-16T20:23:19Z","timestamp":1076962999000},"page":"H22-H28","source":"Crossref","is-referenced-by-count":8,"title":["Flow-dependent increase of ICAM-1 on saphenous vein endothelium is sensitive to apamin"],"prefix":"10.1152","volume":"287","author":[{"given":"Sabena","family":"Sultan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Gosling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shadi","family":"Abu-Hayyeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nessa","family":"Carey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet T.","family":"Powell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Alevriadou BR, Eskin SG, McIntire LV, and Schilling WP. Effect of shear stress on Rb++ efflux from calf pulmonary artery endothelial cells. Ann Biomed Eng 21: 1\u20137, 1993.","DOI":"10.1007\/BF02368159"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02623896"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(97)87271-3"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(01)00476-X"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704551"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1067\/mva.2001.115571"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703188"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/S0741-5214(94)70208-X"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119461"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.33.21084"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(01)00519-3"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.20.3.652"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1007\/BF00374386"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1998.0873"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.145.1.183"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.103.15.1992"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6949"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.6.1841"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.264.4.C1037"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2001.81.4.1415"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117787"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.88.1.193"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.73.3.492"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1038\/331168a0"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1021\/bp970029f"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4598(199906)22:6<742::AID-MUS11>3.0.CO;2-1"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2002.282.1.G116"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106981200"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.269.6.C1371"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00880.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T15:38:10Z","timestamp":1567957090000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpheart.00880.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,7]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,7]]}},"alternative-id":["10.1152\/ajpheart.00880.2003"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00880.2003","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,7]]}}}