{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T20:09:08Z","timestamp":1791835748595,"version":"4.3.4"},"reference-count":48,"publisher":"American Physiological Society","issue":"1","funder":[{"DOI":"10.13039\/100006108","name":"HHS | NIH | National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UM1 TR004399"],"award-info":[{"award-number":["UM1 TR004399"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["24TPA1301309"],"award-info":[{"award-number":["24TPA1301309"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2026,1,1]]},"abstract":"<jats:p>Obesity is associated with profound impairment in the capacity of the vascular endothelium to release tissue-type plasminogen activator (t-PA), the primary mechanism underlying endogenous thrombolysis. Circulating endothelial cell-derived extracellular vesicles (EMVs) have been linked to endothelial dysfunction. This study demonstrates that circulating EMVs are elevated in adults with obesity and are associated with reduced endothelial t-PA release. Circulating EMVs represent a novel systemic biomarker of obesity-related endothelial fibrinolytic dysfunction and, in turn, thrombotic risk.<\/jats:p>","DOI":"10.1152\/ajpheart.00885.2025","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T01:10:20Z","timestamp":1766106620000},"page":"H319-H326","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Obesity-related elevation in circulating endothelial-derived extracellular microvesicles and endothelial fibrinolytic dysfunction"],"prefix":"10.1152","volume":"330","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8040-918X","authenticated-orcid":false,"given":"Samuel T.","family":"Ruzzene","sequence":"first","affiliation":[{"name":"University of Colorado Boulder","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Auburn R.","family":"Berry","sequence":"additional","affiliation":[{"name":"University of Colorado Boulder","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3022-1616","authenticated-orcid":false,"given":"Vinicius P.","family":"Garcia","sequence":"additional","affiliation":[{"name":"University of Colorado Boulder","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Whitney B.","family":"Valenti","sequence":"additional","affiliation":[{"name":"University of Colorado Boulder","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o E.","family":"Izaias","sequence":"additional","affiliation":[{"name":"University of Colorado Boulder","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4855-2202","authenticated-orcid":false,"given":"Kelly A.","family":"Stockelman","sequence":"additional","affiliation":[{"name":"University of Colorado Boulder","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jared J.","family":"Greiner","sequence":"additional","affiliation":[{"name":"University of Colorado Boulder","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0249-5353","authenticated-orcid":false,"given":"Andrew J.","family":"Park","sequence":"additional","affiliation":[{"name":"Craig Hospital","place":["United States"]},{"name":"University of Colorado Anschutz Medical Center","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian L.","family":"Stauffer","sequence":"additional","affiliation":[{"name":"University of Colorado Boulder","place":["United States"]},{"name":"University of Colorado Anschutz Medical Center","place":["United States"]},{"name":"Denver Health Medical Center","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9372-5083","authenticated-orcid":false,"given":"Christopher A.","family":"DeSouza","sequence":"additional","affiliation":[{"name":"University of Colorado Boulder","place":["United States"]},{"name":"University of Colorado Anschutz Medical Center","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.121.318093"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMsa1909301"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.111.639922"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1001\/jama.282.16.1523"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000075294.98582.2F"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000018121.67607.ce"},{"key":"B7","volume-title":"Clinical Guidelines on the Identification, Evaluation and Treatment of Overweight and Obese Adults-The Evidence Report","author":"NHLBI Obesity Education Initiative Expert Panel on the Identification, Evaluation, and Treatment of Obesity in Adults (US)","year":"1998"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/s0002-9149(01)01720-9"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.arcmed.2005.03.013"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/s0002-9343(98)00209-5"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1177\/1358863X9500600208"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V85.12.3510.bloodjournal85123510"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.3.1976"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2014.04.007"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00072.2005"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2011.189"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2025.118415"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.2174\/1874192401004010302"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2008.080228"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2005050535"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2015.02.003"},{"key":"B22","first-page":"116","volume":"8","author":"Berezin AE","year":"2014","journal-title":"Int Cardiovasc Res J"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1177\/1358863X13499773"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.310752"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.2147\/DMSO.S32923"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1160\/TH16-03-0176"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/s0735-1097(02)02060-0"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.99.11.1411"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00139.2023"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.044107"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.027870"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1161\/01.atv.17.12.3376"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V7.12.1227.1227"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1161\/01.str.27.11.2005"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00081.2025"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000189309.05924.88"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.14534"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1182\/blood.v99.11.3962"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1097\/MED.0b013e3282be90a8"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2020.1790426"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2020.104422"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.3233\/CH-209007"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.2174\/1574888X11666151203224058"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00243.2023"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.103.15.1936"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.3390\/biom10020291"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1161\/hc4701.100516"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacbts.2017.08.004"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00885.2025","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T16:14:46Z","timestamp":1767888886000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpheart.00885.2025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,1]]},"references-count":48,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1,1]]}},"alternative-id":["10.1152\/ajpheart.00885.2025"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00885.2025","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,1]]},"assertion":[{"value":"2025-11-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-24","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}