{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T05:00:54Z","timestamp":1761541254606},"reference-count":33,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2002,4,1]]},"abstract":"<jats:p> We have compared the mechanisms that contribute to endothelium-derived hyperpolarizing factor (EDHF)-type responses induced by ACh and the Ca<jats:sup>2+<\/jats:sup> ionophore A-23187 in the rabbit iliac artery. Relaxations to both agents were associated with \u223c1.5-fold elevations in smooth muscle cAMP levels and were attenuated by the adenylyl cyclase inhibitor 2\u2032,5\u2032-dideoxyadenosine (DDA) and potentiated by the cAMP phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX). Mechanical responses were inhibited by coadministration of the Ca<jats:sup>2+<\/jats:sup>-activated K<jats:sup>+<\/jats:sup>channel blockers apamin and charybdotoxin, both in the absence and presence of IBMX, but were unaffected by blockade of ATP-sensitive K<jats:sup>+<\/jats:sup> channels with the sulphonylurea glibenclamide. Relaxations and elevations in cAMP evoked by ACh were abolished by 18\u03b1-glycyrrhetinic acid, which disrupts gap junction plaques, whereas the corresponding responses to A-23187 were unaffected by this agent. Consistently, in \u201csandwich\u201d bioassay experiments, A-23187, but not ACh, elicited extracellular release of a factor that evoked relaxations that were inhibited by DDA and potentiated by IBMX. These findings provide evidence that EDHF-type relaxations of rabbit iliac arteries evoked by ACh and A-23187 depend on cAMP accumulation in smooth muscle, but involve signaling via myoendothelial gap junctions and the extracellular space, respectively. <\/jats:p>","DOI":"10.1152\/ajpheart.00903.2001","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T15:02:11Z","timestamp":1425394931000},"page":"H1548-H1555","source":"Crossref","is-referenced-by-count":26,"title":["Gap junction-dependent and -independent EDHF-type relaxations may involve smooth muscle cAMP accumulation"],"prefix":"10.1152","volume":"282","author":[{"given":"Andrew T.","family":"Chaytor","sequence":"first","affiliation":[{"name":"Department of Diagnostic Radiology, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff CF14 4XN, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah 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