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Vasospasm was assessed by angiography performed before and 4, 7, or 21 days after SAH. Basilar arteries were excised from SAH or control dogs ( n = 8\u201352 arterial rings from 2\u20139 dogs per measurement) and studied under isometric tension in vitro before and after permeabilization of smooth muscle with \u03b1-toxin. Endothelium was removed from all arteries. Vasospastic arteries demonstrated significantly reduced contractility to KCl with a shift in the EC<jats:sub>50<\/jats:sub>toward reduced sensitivity to KCl 4 and 7 days after SAH ( P &lt; 0.05, ANOVA). There was reduced compliance that persisted after permeabilization ( P &lt; 0.05, ANOVA). Calcium sensitivity was decreased during vasospasm 4 and 7 days after SAH, as assessed in permeabilized arteries and in those contracted with BAY K 8644 in the presence of different concentrations of extracellular calcium ( P &lt; 0.05, ANOVA). Depolymerization of actin with cytochalasin D abolished contractions to KCl but failed to alter arterial compliance. In conclusion, it is shown for the first time that calcium sensitivity is decreased during vasospasm after SAH in dogs, suggesting that other mechanisms are involved in maintaining the contraction. Reduced compliance seems to be due to an alteration in the arterial wall extracellullar matrix rather than the smooth muscle cells themselves because it cannot be alleviated by depolymerization of smooth muscle actin.<\/jats:p>","DOI":"10.1152\/ajpheart.00911.2005","type":"journal-article","created":{"date-parts":[[2006,1,7]],"date-time":"2006-01-07T01:49:24Z","timestamp":1136598564000},"page":"H2329-H2336","source":"Crossref","is-referenced-by-count":16,"title":["Calcium sensitivity of vasospastic basilar artery after experimental subarachnoid hemorrhage"],"prefix":"10.1152","volume":"290","author":[{"given":"R. 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