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The ratio of phosphocreatine concentration to ATP concentration was \u223c2.1. Also, intracellular pH and Mg<jats:sup>2+<\/jats:sup>concentration ([Mg<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>), estimated from the chemical shifts of inorganic phosphate and ATP, were comparable with those under retrograde perfusion. [Mg<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>was significantly increased by the removal of extracellular Na<jats:sup>+<\/jats:sup>, supporting the essential role of Na<jats:sup>+<\/jats:sup>-coupled Mg<jats:sup>2+<\/jats:sup>transport in Mg<jats:sup>2+<\/jats:sup>homeostasis of the heart. Heart slice preparation could also be used to evaluate the potency of cardiac drugs, regardless of their possible effects on coronary microcirculation.<\/jats:p>","DOI":"10.1152\/ajpheart.00923.2005","type":"journal-article","created":{"date-parts":[[2006,10,15]],"date-time":"2006-10-15T06:15:47Z","timestamp":1160892947000},"page":"H1181-H1186","source":"Crossref","is-referenced-by-count":3,"title":["Heart slice 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