{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T15:49:58Z","timestamp":1788536998192,"version":"build-2803163510"},"reference-count":20,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:p> Ascorbate reduces the oxidation rate of catecholamines and, by an independent mechanism, enhances rabbit aortic ring contractions initiated by catecholamines. The largest significantly different fractional increases in force produced by ascorbate enhancement of norepinephrine (NE), epinephrine, phenylpropanolamine (PPA), and ephedrine (Eph) are 5.5, 1.8, 1.6, and 1.3 times, respectively. In physiological salt solutions bubbled with 95% O<jats:sub>2<\/jats:sub> at 37\u00b0C, NE, PPA, and Eph have oxidation rate constants of 1.24, 247, and 643 h, respectively. Ascorbate significantly enhances 100 nM NE contractions by at least twofold at all ascorbate concentrations &gt;15 \u03bcM, including the entire physiological range of 40\u2013100 \u03bcM. Ascorbate preloading and washout followed by NE exposure produces significantly greater contractions than NE without ascorbate preloading but significantly lower than NE simultaneously with ascorbate. Ascorbate does not enhance K<jats:sup>+<\/jats:sup>- or angiotensin II-induced contractions. Ascorbate enhancement of catecholamine contractions occurs in addition to the reduction in oxidation rate, because the increases in force occur faster than oxidation can occur, the increases occur with compounds that have negligible oxidation rates, and the increases occur when ascorbate and NE are not physically present together. These results are consistent with ascorbate acting on the adrenergic receptor. Ascorbate may play a role in shock and asthma treatments and potentiate the cardiovascular health consequences of PPA and Eph (Ephedra). <\/jats:p>","DOI":"10.1152\/ajpheart.00968.2003","type":"journal-article","created":{"date-parts":[[2004,2,24]],"date-time":"2004-02-24T01:50:34Z","timestamp":1077587434000},"page":"H2353-H2360","source":"Crossref","is-referenced-by-count":43,"title":["Antioxidant-independent ascorbate enhancement of catecholamine-induced contractions of vascular smooth muscle"],"prefix":"10.1152","volume":"286","author":[{"given":"P. F.","family":"Dillon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. S.","family":"Root-Bernstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. M.","family":"Lieder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","unstructured":"Altman PL. In: Blood and Other Body Fluids, edited by Dittmer DS. Washington, DC: FASEB, 1961, p. 89\u201390."},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01945566"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.31.3.854"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(00)76298-9"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(84)90315-1"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.2500\/105065801781543772"},{"key":"R7","unstructured":"Furchgott RF. The pharmacology of vascular smooth muscle. Pharmacol Rev 7: 183\u2013265, 1956."},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg563"},{"key":"R9","unstructured":"Hoffman BF and Lefkowitz RJ. Catecholamine and sympathomimetic drugs. In: Goodman and Gilman's The Pharmacological Basis of Therapeutics (8th ed.), edited by Gilman AG, Rall TW, Nies AS, and Taylor P. New York: Pergamon, 1990, p. 187\u2013220."},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-1838(96)00084-7"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200012213432501"},{"key":"R12","doi-asserted-by":"crossref","unstructured":"Kitto HJ and Bohr DF. Some agents which potentiate the inotropic response of papillary muscle to epinephrine. Am J Physiol 175: 343\u2013348, 1953.","DOI":"10.1152\/ajplegacy.1953.175.3.343"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1016\/0026-2862(83)90057-2"},{"key":"R14","unstructured":"Otomo S, Kubo J, Mihara B, Gomi S, and Suga S. A case with cerebral subcortical hemorrhage following the administration of phenylpropanolamine. Rinsho Shinkeigaku 41: 407\u2013411, 2001."},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2143(02)00020-3"},{"key":"R16","doi-asserted-by":"crossref","unstructured":"Rasmussen H, Barrett P, Takuwa P, and Apfeldorf W. Calcium in the regulation of aldosterone secretion and vascular smooth muscle contraction. Hypertension 10: 123\u2013126, 1987.","DOI":"10.1161\/01.HYP.10.5_Pt_2.I23"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2396(20010101)39:1<32::AID-SYN5>3.0.CO;2-3"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1006\/fgbi.2001.1324"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-1280(98)90217-2"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1002\/j.1552-4604.1990.tb03608.x"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00968.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T19:39:45Z","timestamp":1567971585000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpheart.00968.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6]]},"references-count":20,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2004,6]]}},"alternative-id":["10.1152\/ajpheart.00968.2003"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00968.2003","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,6]]}}}