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The purpose of our investigation was to examine the direct effects of PGF<jats:sub>2\u03b1<\/jats:sub>on membrane potentials, Ca<jats:sup>2+<\/jats:sup>-activated K<jats:sup>+<\/jats:sup>(K<jats:sub>Ca<\/jats:sub>) channels, delayed rectifier K<jats:sup>+<\/jats:sup>(K<jats:sub>V<\/jats:sub>) channels, and L-type Ca<jats:sup>2+<\/jats:sup>channels with the patch-clamp technique in single rabbit middle cerebral arterial smooth muscle cells (SMCs). PGF<jats:sub>2\u03b1<\/jats:sub>significantly hyperpolarized membrane potentials and increased the amplitudes of total K<jats:sup>+<\/jats:sup>currents. PGF<jats:sub>2\u03b1<\/jats:sub>increased open-state probability but had little effect on the open and closed kinetics of K<jats:sub>Ca<\/jats:sub>channels. PGF<jats:sub>2\u03b1<\/jats:sub>increased the amplitudes of K<jats:sub>V<\/jats:sub>currents with a leftward shift of the activation and inactivation curves and a decrease in the activation time constant. PGF<jats:sub>2\u03b1<\/jats:sub>decreased the amplitudes of L-type Ca<jats:sup>2+<\/jats:sup>currents without any significant change in threshold or apparent reversal potentials. This study provides the first finding that the direct effects of PGF<jats:sub>2\u03b1<\/jats:sub>on middle cerebral arterial SMCs, at least in part, could attenuate vasoconstriction.<\/jats:p>","DOI":"10.1152\/ajpheart.01022.2001","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:07:55Z","timestamp":1425413275000},"page":"H1018-H1027","source":"Crossref","is-referenced-by-count":4,"title":["Effects of prostaglandin F<sub>2\u03b1<\/sub>on membrane currents in rabbit middle cerebral arterial smooth muscle cells"],"prefix":"10.1152","volume":"284","author":[{"given":"Nari","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, College of Medicine, Inje University, Busanjin-gu, Busan, 614-735, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, College of Medicine, Inje University, 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