{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:44:28Z","timestamp":1791074668566,"version":"4.1.0"},"reference-count":43,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:p> In a rat model of long-lasting pressure-overload hypertrophy, we investigated whether changes in the relative expression of myocardial actin isoforms are among the early signs of ventricular mechanical dysfunction before the transition toward decompensation. Forty-four rats with infrarenal aortic banding (AC rats) were studied. Hemodynamic parameters were measured 1 mo (AC<jats:sub>1<\/jats:sub> group; n = 20) or 2 mo (AC<jats:sub>2<\/jats:sub>; n = 24) after aortic ligature. Then subgroups of AC<jats:sub>1<\/jats:sub> and AC<jats:sub>2<\/jats:sub> left ventricles (LV) were used to evaluate 1) LV anatomy and fibrosis (morphometry), 2) expression levels (immunoblotting) and spatial distribution (immunohistochemistry) of alpha-skeletal actin (\u03b1-SKA), alpha-cardiac actin (\u03b1-CA), and alpha-smooth muscle actin (\u03b1-SMA), and 3) cell mechanics and calcium transients in enzimatically isolated myocytes. Although the two AC groups exhibited a comparable degree of hypertrophy (+30% in LV mass; +20% in myocyte surface) and a similar increase in the amount of fibrosis compared with control animals (C group; n = 22), a worsening of LV mechanical performance was observed only in AC<jats:sub>2<\/jats:sub> rats at both organ and cellular levels. Conversely, AC<jats:sub>1<\/jats:sub> rats exhibited enhanced LV contractility and preserved cellular contractile behavior associated with increased calcium transients. Alpha-SKA expression was upregulated (+60%) in AC<jats:sub>1<\/jats:sub>. In AC<jats:sub>2<\/jats:sub> ventricles, prolonged hypertension also induced a significant increase in \u03b1-SMA expression, mainly at the level of arterial vessels. No significant differences among groups were observed in \u03b1-CA expression. Our findings suggest that \u03b1-SKA expression regulation and wall remodeling of coronary arterioles participate in the development of impaired kinetics of contraction and relaxation in prolonged hypertension before the occurrence of marked histopathologic changes. <\/jats:p>","DOI":"10.1152\/ajpheart.01057.2008","type":"journal-article","created":{"date-parts":[[2009,2,28]],"date-time":"2009-02-28T03:08:26Z","timestamp":1235790506000},"page":"H1625-H1632","source":"Crossref","is-referenced-by-count":20,"title":["Modulation of actin isoform expression before the transition from experimental compensated pressure-overload cardiac hypertrophy to decompensation"],"prefix":"10.1152","volume":"296","author":[{"given":"Roberta","family":"Berni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monia","family":"Savi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Bocchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Delucchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ezio","family":"Musso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Chaponnier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulio","family":"Gabbiani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Clement","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donatella","family":"Stilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-005-1490-7"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1996.0149"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000056601.29613.DD"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01345.2005"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00104.2004"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00259.2007"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.85.10.e51"},{"key":"R8","unstructured":"Clement S, Orlandi A, Bocchi L, Pizzolato G, Foschini MP, Eusebi V, Gabbiani G. Actin isoform pattern expression: a tool for the diagnosis and biological characterization of human rhabdomyosarcoma. Virchows Arch 442: 31\u201338, 2003."},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9149(69)90006-X"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1161\/hy1201.099615"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcad.2006.02.001"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00355.2006"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(99)00268-0"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.74.4.740"},{"key":"R15","doi-asserted-by":"crossref","unstructured":"Higashiyama H, Sugai M, Inoue H, Mizuyachi K, Kushida H, Asano S, Kinoshita M. Histopathological study of time course changes in inter-renal aortic banding-induced left ventricular hypertrophy of mice. Int J Exp Pathol 88: 31\u201338, 2007.","DOI":"10.1111\/j.1365-2613.2006.00514.x"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.070112"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1161\/hh1701.095522"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1136\/heart.87.4.368"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000020689.12472.E0"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1054\/jcaf.2002.129276"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500013"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2006.05.065"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.81.1.260"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.013415"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68074-4"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00437.2004"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1249\/01.mss.0000240324.08406.3d"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.151449"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000161873.27088.4c"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070145"},{"key":"R32","unstructured":"Schurch W, Skalli O, Seemayer TA, Gabbiani G. Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. I. Smooth muscle tumors. Am J Pathol 128: 91\u2013103, 1987."},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.59.5.551"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2006.04.004"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.6.2787"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2005.032607"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1002\/path.1311"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1007\/s10741-005-5250-8"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1097\/00001573-200007000-00010"},{"key":"R41","unstructured":"Willems IE, Havenith MG, De Mey JG, Daemen MJ. The alpha-smooth muscle actin-positive cells in healing human myocardial scars. Am J Pathol 145: 868\u2013875, 1994."},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-200502000-00022"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.278.3.H677"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpheart.01057.2008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T21:21:34Z","timestamp":1567977694000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpheart.01057.2008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,5]]},"references-count":43,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2009,5]]}},"alternative-id":["10.1152\/ajpheart.01057.2008"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.01057.2008","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,5]]}}}