{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:25:47Z","timestamp":1791350747489,"version":"4.3.1"},"reference-count":45,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:p>Although ischemia-reperfusion (I\/R) has been shown to affect subcellular organelles that regulate the intracellular Ca<jats:sup>2+<\/jats:sup>concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>), very little information regarding the Ca<jats:sup>2+<\/jats:sup>handling ability of cardiomyocytes obtained from I\/R hearts is available. To investigate changes in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>due to I\/R, rat hearts in vitro were subjected to 10\u201330 min of ischemia followed by 5\u201330 min of reperfusion. Cardiomyocytes from these hearts were isolated and purified; [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>was measured by employing fura-2 microfluorometry. Reperfusion for 30 min of the 20-min ischemic hearts showed attenuated cardiac performance, whereas basal [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>as well as the KCl-induced increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>and isoproterenol (Iso)-induced increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in cardiomyocytes remained unaltered. On the other hand, reperfusion of the 30-min ischemic hearts for different periods revealed marked changes in cardiac function, basal [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>, and Iso-induced increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>without any alterations in the KCl-induced increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>or S(\u2212)-BAY K 8644-induced increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. The I\/R-induced alterations in cardiac function, basal [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>, and Iso-induced increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in cardiomyocytes were attenuated by an antioxidant mixture containing superoxide dismutase and catalase as well as by ischemic preconditioning. The observed changes due to I\/R were simulated in hearts perfused with H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>for 30 min. These results suggest that abnormalities in basal [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>as well as mobilization of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>upon \u03b2-adrenoceptor stimulation in cardiomyocytes are dependent on the duration of ischemic injury to the myocardium. Furthermore, Ca<jats:sup>2+<\/jats:sup>handling defects in cardiomyocytes appear to be mediated through oxidative stress in I\/R hearts.<\/jats:p>","DOI":"10.1152\/ajpheart.01153.2004","type":"journal-article","created":{"date-parts":[[2005,1,15]],"date-time":"2005-01-15T01:15:21Z","timestamp":1105751721000},"page":"H2260-H2270","source":"Crossref","is-referenced-by-count":58,"title":["Defective calcium handling in cardiomyocytes isolated from hearts subjected to ischemia-reperfusion"],"prefix":"10.1152","volume":"288","author":[{"given":"Harjot K.","family":"Saini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naranjan 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