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In this study, we tested for metabolic and functional alterations in the heart from 3- and 7-mo-old VLCAD null mice and their littermate counterparts, using validated experimental paradigms, namely, 1) ex vivo perfusion in working mode, with concomitant evaluation of myocardial contractility and metabolic fluxes using<jats:sup>13<\/jats:sup>C-labeled substrates under various conditions; as well as 2) in vivo targeted lipidomics, gene expression analysis as well as electrocardiogram monitoring by telemetry in mice fed various diets. Unexpectedly, when perfused ex vivo, working VLCAD null mouse hearts maintained values similar to those of the controls for functional parameters and for the contribution of exogenous palmitate to \u03b2-oxidation (energy production), even at high palmitate concentration (1 mM) and increased energy demand (with 1 \u03bcM epinephrine) or after fasting. However, in vivo, these hearts displayed a prolonged rate-corrected QT (QTc) interval under all conditions examined, as well as the following lipid alterations: 1) age- and condition-dependent accumulation of triglycerides, and 2) 20% lower docosahexaenoic acid (an omega-3 polyunsaturated fatty acid) in membrane phospholipids. The latter was independent of liver but affected by feeding a diet enriched in saturated fat (exacerbated) or fish oil (attenuated). Our finding of a longer QTc interval in VLCAD null mice appears to be most relevant given that such condition increases the risk of sudden cardiac death.<\/jats:p>","DOI":"10.1152\/ajpheart.01275.2010","type":"journal-article","created":{"date-parts":[[2011,6,18]],"date-time":"2011-06-18T04:17:50Z","timestamp":1308370670000},"page":"H813-H823","source":"Crossref","is-referenced-by-count":48,"title":["Prolonged QT interval and lipid alterations beyond \u03b2-oxidation in very long-chain acyl-CoA dehydrogenase null mouse hearts"],"prefix":"10.1152","volume":"301","author":[{"given":"Roselle","family":"G\u00e9linas","sequence":"first","affiliation":[{"name":"Department of Nutrition, Universit\u00e9 de Montr\u00e9al, and"},{"name":"Montreal Heart Institute, Montreal, Quebec, Canada; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Thompson-Legault","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Universit\u00e9 de Montr\u00e9al, 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