{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:42:11Z","timestamp":1792676531633,"version":"4.5.0"},"reference-count":40,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:p>\n                    Caveolae are \u03a9-shaped vesicular structures postulated to play a role in transvascular protein transport. Studies on mice lacking endothelial caveolae, caveolin-1 knockout (Cav-1-KO) mice, indicate increased macromolecular transport rates. This was postulated to be due to the appearance of an alternative pathway. The present study tested whether an alternative pathway had appeared in Cav-1-KO mice. Male Cav-1-KO ( n = 12) and male control mice ( n = 13) were intubated and anesthetized using 2% isoflurane.\n                    <jats:sup>125<\/jats:sup>\n                    I-labeled albumin,\n                    <jats:sup>131<\/jats:sup>\n                    I-labeled immunoglobulin M (IgM), and polydisperse FITC-Ficoll were administered intravenously. During tracer administration, a 90-min peritoneal dialysis dwell was performed. Clearance of tracers to dialysate and permeability-surface area product for glucose were assessed. Transvascular protein transport was higher in Cav-1-KO compared with control mice. Albumin clearance from plasma to peritoneum was 0.088 \u00b1 0.008 \u03bcl\/min in control and 0.179 \u00b1 0.012 \u03bcl\/min in Cav-1-KO ( P = 0.001) mice. IgM clearance was 0.049 \u00b1 0.003 and 0.083 \u00b1 0.010 \u03bcl\/min in control and Cav-1-KO mice, respectively ( P = 0.016). Ficoll clearance was increased in Cav-1-KO mice. In conclusion, the lack of caveolae in Cav-1-KO mice resulted in a marked increase in macromolecular transport. A two-pore analysis of the Ficoll clearance data revealed that the higher transport rate in Cav-1-KO mice was not compatible with the appearance of an alternative pathway for macromolecular transport. In contrast, the higher transperitoneal protein and Ficoll clearance is consistent with passive porous transport through an unperturbed two-pore system, presumably at an elevated capillary hydraulic pressure. Alternatively, the data may be explained by reductions in the selectivity of the endothelial glycocalyx, leading to an increased capillary hydraulic conductivity and large solute filtration.\n                  <\/jats:p>","DOI":"10.1152\/ajpheart.01364.2005","type":"journal-article","created":{"date-parts":[[2006,2,24]],"date-time":"2006-02-24T20:34:16Z","timestamp":1140813256000},"page":"H1371-H1377","source":"Crossref","is-referenced-by-count":56,"title":["Transvascular protein transport in mice lacking endothelial caveolae"],"prefix":"10.1152","volume":"291","author":[{"given":"Bert-Inge","family":"Rosengren","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Rippe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catarina","family":"Rippe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Venturoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl","family":"Sw\u00e4rd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bengt","family":"Rippe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(84)90177-1"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1996.271.6.H2254"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-201x.2000.00685.x"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2001.281.4.H1728"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1080\/10739680590894993"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1126\/science.1062688"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-004-3445-0"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/0022-5320(80)90111-2"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.24.9265"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.3109\/10739689509146769"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1980.sp013512"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1999.79.3.703"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1109"},{"key":"R14","unstructured":"Palade GE\n                      .\n                      Fine structure of blood capillaries.\n                      J Appl Physiol\n                      24\n                      : 1424\u20131424, 1953."},{"key":"R15","unstructured":"Palade GE\n                      .\n                      Transport in quanta across the endothelium of blood capillaries.\n                      Anat Rec\n                      136\n                      : 254\u2013254, 1960."},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1038\/327524a0"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1159\/000075677"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1080\/713773617"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.8.3014"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1993.265.2.H725"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.11.6175"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1997.272.2.H937"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1994.74.1.163"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1979.tb06329.x"},{"key":"R25","unstructured":"Rippe B\n                      and Krediet RT.\n                      Peritoneal physiology-Transport of solutes. In:\n                      The Textbook of Peritoneal Dialysis\n                      (1st ed.), edited by Gokal R and Nolph KD. Dordrecht: Kluwer Academic, 1994, p. 69\u2013113."},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.00114.x"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1159\/000064521"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1549-8719.2001.tb00178.x"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1991.216"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2001.280.1.H34"},{"key":"R31","doi-asserted-by":"crossref","unstructured":"Rippe B\n                      , Venturoli D, Simonsen O, and de Arteaga J.\n                      Fluid and electrolyte transport across the peritoneal membrane during CAPD according to the three-pore model.\n                      Perit Dial Int\n                      24\n                      : 10\u201327, 2004.","DOI":"10.1177\/089686080402400102"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1159\/000063688"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1159\/000077131"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000065551.75164.06"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1992.262.1.H234"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1995.268.5.H1982"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1995.268.1.H48"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.127.5.1217"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205948200"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1159\/000170209"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpheart.01364.2005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,4,12]],"date-time":"2020-04-12T18:35:20Z","timestamp":1586716520000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpheart.01364.2005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,9]]}},"alternative-id":["10.1152\/ajpheart.01364.2005"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.01364.2005","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1021316.371725","asserted-by":"object"}]},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,9]]}}}