{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:38:28Z","timestamp":1761129508448,"version":"3.41.0"},"reference-count":44,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2000,2,1]]},"abstract":"<jats:p>Overexpression of calcitonin gene-related peptide (CGRP), an extremely potent vasodilator, to blood vessels is a possible strategy for prevention of vasospasm. We constructed an adenoviral vector that encodes prepro-CGRP (Adprepro-CGRP) and examined the effects of gene transfer on cultured cells and cerebral arteries. Transfection of Adprepro-CGRP to Cos-7 and NIH-3T3 cells increased CGRP-like immunoreactivity in media and produced an increase in cAMP in recipient cells. Five days after injection of Adprepro-CGRP into the cisterna magna of rabbits, the concentration of CGRP-like immunoreactivity increased by 93-fold in cerebrospinal fluid. In basilar artery, cAMP increased by 2.3-fold after Adprepro-CGRP compared with a control adenovirus. After transfection of Adprepro-CGRP, contraction of basilar artery in vitro to histamine and serotonin was attenuated, and relaxation to an inhibitor of cyclic nucleotide phosphodiesterase 3-isobutyl-1-methylxanthine was augmented compared with nontransduced arteries or arteries transfected with a control gene. Altered vascular responses were restored to normal by pretreatment with a CGRP<jats:sub>1<\/jats:sub>receptor antagonist CGRP-(8\u201337). Thus gene transfer of prepro-CGRP in vivo overexpresses CGRP in cerebrospinal fluid and perivascular tissues and modulates vascular tone. We speculate that this approach may be useful in prevention of vasospasm after subarachnoid hemorrhage.<\/jats:p>","DOI":"10.1152\/ajpheart.2000.278.2.h586","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T06:52:44Z","timestamp":1513925564000},"page":"H586-H594","source":"Crossref","is-referenced-by-count":31,"title":["Gene transfer of calcitonin gene-related peptide to cerebral arteries"],"prefix":"10.1152","volume":"278","author":[{"given":"Kazunori","family":"Toyoda","sequence":"first","affiliation":[{"name":"Departments of Internal Medicine and Pharmacology, Cardiovascular Center, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank M.","family":"Faraci","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine and Pharmacology, Cardiovascular Center, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew F.","family":"Russo","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Iowa College of Medicine, and Veterans Administration Medical Center, Iowa City, Iowa 52242"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beverly L.","family":"Davidson","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine and Pharmacology, Cardiovascular Center, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald D.","family":"Heistad","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine and Pharmacology, Cardiovascular Center, and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"doi-asserted-by":"publisher","key":"B1","DOI":"10.1038\/nm0696-696"},{"doi-asserted-by":"publisher","key":"B2","DOI":"10.1038\/298240a0"},{"doi-asserted-by":"publisher","key":"B3","DOI":"10.1016\/0165-6147(90)90066-H"},{"doi-asserted-by":"publisher","key":"B4","DOI":"10.1038\/313054a0"},{"doi-asserted-by":"publisher","key":"B5","DOI":"10.1074\/jbc.272.24.15481"},{"doi-asserted-by":"publisher","key":"B6","DOI":"10.1161\/01.RES.80.3.327"},{"doi-asserted-by":"publisher","key":"B7","DOI":"10.1073\/pnas.94.23.12568"},{"doi-asserted-by":"publisher","key":"B8","DOI":"10.1126\/science.275.5301.838"},{"doi-asserted-by":"publisher","key":"B9","DOI":"10.1161\/01.STR.29.7.1411"},{"doi-asserted-by":"publisher","key":"B10","DOI":"10.1016\/0306-4522(92)90237-V"},{"doi-asserted-by":"publisher","key":"B11","DOI":"10.1016\/0304-3940(91)90672-G"},{"doi-asserted-by":"publisher","key":"B12","DOI":"10.1016\/0304-3940(85)90166-1"},{"key":"B13","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1016\/S0022-3565(25)24738-3","volume":"257","author":"Edwards R. 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