{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T10:59:11Z","timestamp":1772794751212,"version":"3.50.1"},"reference-count":49,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2001,2,1]]},"abstract":"<jats:p>The activities of Na-K-ATPase and Na-K-2Cl cotransporter (NKCC1) were studied in the aorta, heart, and skeletal muscle of streptozotocin (STZ)-induced diabetic rats and control rats. In the aortic rings of STZ rats, the Na-K-ATPase-dependent<jats:sup>86<\/jats:sup>Rb\/K uptake was reduced to 60.0 \u00b1 5.5% of the control value ( P &lt; 0.01). However, Na-K-ATPase activity in soleus skeletal muscle fibers of STZ rats and paired control rats was similar, showing that the reduction of Na-K-ATPase activity in aortas of STZ rats is tissue specific. To functionally distinguish the contributions of ouabain-resistant (\u03b1<jats:sub>1<\/jats:sub>) and ouabain-sensitive (\u03b1<jats:sub>2<\/jats:sub>and \u03b1<jats:sub>3<\/jats:sub>) isoforms to the Na-K-ATPase activity in aortic rings, we used either a high (10<jats:sup>\u22123<\/jats:sup>M) or a low (10<jats:sup>\u22125<\/jats:sup>M) ouabain concentration during<jats:sup>86<\/jats:sup>Rb\/K uptake. We found that the reduction in total Na-K-ATPase activity resulted from a dramatic decrement in ouabain-sensitive mediated<jats:sup>86<\/jats:sup>Rb\/K uptake (26.0 \u00b1 3.9% of control, P &lt; 0.01). Western blot analysis of membrane fractions from aortas of STZ rats demonstrated a significant reduction in protein levels of \u03b1<jats:sub>1<\/jats:sub>- and \u03b1<jats:sub>2<\/jats:sub>-catalytic isoforms (\u03b1<jats:sub>1<\/jats:sub>= 71.3 \u00b1 9.8% of control values, P &lt; 0.05; \u03b1<jats:sub>2<\/jats:sub>= 44.5 \u00b1 11.3% of control, P &lt; 0.01). In contrast, aortic rings from the STZ rats demonstrated an increase in NKCC1 activity (172.5 \u00b1 9.5%, P &lt; 0.01); however, in heart tissue no difference in NKCC1 activity was seen between control and diabetic animals. Transport studies of endothelium-denuded or intact aortic rings demonstrated that the endothelium stimulates both Na-K-ATPase and Na-K-2Cl dependent<jats:sup>86<\/jats:sup>Rb\/K uptake. The endothelium-dependent stimulation of Na-K-ATPase and Na-K-2Cl was not hampered by diabetes. We conclude that abnormal vascular vessel tone and function, reported in STZ-induced diabetic rats, may be related to ion transport abnormalities caused by changes in Na-K-ATPase and Na-K-2Cl activities.<\/jats:p>","DOI":"10.1152\/ajpheart.2001.280.2.h851","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T08:49:14Z","timestamp":1513932554000},"page":"H851-H858","source":"Crossref","is-referenced-by-count":18,"title":["Reduced Na-K pump but increased Na-K-2Cl cotransporter in aorta of streptozotocin-induced diabetic rat"],"prefix":"10.1152","volume":"280","author":[{"given":"Luis","family":"Michea","sequence":"first","affiliation":[{"name":"National Institutes of Health, Bethesda, Maryland 20892-1603"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f3nica","family":"Irribarra","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Physiology, Faculty of Medicine, University of Los Andes, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. 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