{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:16:52Z","timestamp":1789755412318,"version":"4.0.1"},"reference-count":33,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2001,4,1]]},"abstract":"\n Uterine blood flow (UBF) and uterine artery endothelial nitric oxide synthase (eNOS) expression are greatest during the follicular vs. luteal phase. 17\u03b2-Estradiol (E\n 2<\/jats:sub>\n \u03b2) increases UBF and elevates eNOS in ovine uterine but not systemic arteries; progesterone (P\n 4<\/jats:sub>\n ) effects on E\n 2<\/jats:sub>\n \u03b2 changes of eNOS remain unclear. Nonpregnant ovariectomized sheep received either vehicle ( n = 10), P\n 4<\/jats:sub>\n (0.9 g Controlled Internal Drug Release vaginal implants; n = 13), E\n 2<\/jats:sub>\n \u03b2 (5 \u03bcg\/kg bolus + 6 \u03bcg \u00b7 kg\n \u22121<\/jats:sup>\n \u00b7 day\n \u22121<\/jats:sup>\n ; n = 10), or P\n 4<\/jats:sub>\n + E\n 2<\/jats:sub>\n \u03b2 ( n = 12). Reproductive (uterine\/mammary) and nonreproductive (omental\/renal) artery endothelial proteins were procured on day 10, and eNOS was measured by Western analysis. P\n 4<\/jats:sub>\n and E\n 2<\/jats:sub>\n \u03b2 alone and in combination increased ( P < 0.05) eNOS expression in uterine artery endothelium (vehicle = 100 \u00b1 16%, P\n 4<\/jats:sub>\n = 251 \u00b1 59%, E\n 2<\/jats:sub>\n \u03b2 = 566 \u00b1 147%, P\n 4<\/jats:sub>\n + E\n 2<\/jats:sub>\n \u03b2 = 772 \u00b1 211% of vehicle). Neither omental, renal, nor mammary artery eNOS was altered, demonstrating the local nature of steroid-induced maintenance of uterine arterial eNOS. In the myometrial microvasculature, eNOS was increased slightly ( P = 0.06) with E\n 2<\/jats:sub>\n \u03b2 and significantly with P\n 4<\/jats:sub>\n + E\n 2<\/jats:sub>\n \u03b2. Systemic NO\n x<\/jats:sub>\n was increased with P\n 4<\/jats:sub>\n and P\n 4<\/jats:sub>\n + E\n 2<\/jats:sub>\n \u03b2, but not E\n 2<\/jats:sub>\n \u03b2, suggesting differential regulation of eNOS expression and activity, since P\n 4<\/jats:sub>\n increased eNOS in uterine artery endothelium while E\n 2<\/jats:sub>\n \u03b2 and the combination further increased eNOS protein.\n <\/jats:p>","DOI":"10.1152\/ajpheart.2001.280.4.h1699","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T13:14:34Z","timestamp":1513948474000},"page":"H1699-H1705","source":"Crossref","is-referenced-by-count":88,"title":["Endothelial vasodilator production by uterine and systemic arteries. VII. 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