{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T10:28:04Z","timestamp":1774952884901,"version":"3.50.1"},"reference-count":38,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2001,7,1]]},"abstract":"<jats:p>Parathyroid hormone-related protein (PTHrP) was originally found as a tumor-derived vasoactive factor and has also been known to produce significant relaxation of vascular smooth muscles. Thus effects of PTHrP-(1-34), a PTH receptor-binding domain, on spontaneous lymphatic pump activity was investigated in isolated pressurized lymph vessels of mice. Low concentrations (1 \u00d7 10<jats:sup>\u221210<\/jats:sup>and 3 \u00d7 10<jats:sup>\u221210<\/jats:sup>M) of PTHrP-(1-34) dilated lymph vessels and reduced the frequency of pump activity, whereas high concentrations (1 \u00d7 10<jats:sup>\u22129<\/jats:sup>to 1 \u00d7 10<jats:sup>\u22128<\/jats:sup>M) of PTHrP-(1-34) caused dilation with cessation of the lymphatic pump activity. N<jats:sup>\u03c9<\/jats:sup>-nitro-l-arginine methyl ester (l-NAME; 3 \u00d7 10<jats:sup>\u22125<\/jats:sup>M) but not indomethacin (1 \u00d7 10<jats:sup>\u22125<\/jats:sup>M) significantly reduced the PTHrP-(1-34)-induced inhibitory responses of the lymphatic pump activity. In the presence of l-NAME (3 \u00d7 10<jats:sup>\u22125<\/jats:sup>M) and l-arginine (1 \u00d7 10<jats:sup>\u22123<\/jats:sup>M), the l-NAME-induced inhibition in the PTHrP-(1-34)-mediated responses was significantly reduced. Glibenclamide (1 \u00d7 10<jats:sup>\u22126<\/jats:sup>M) significantly suppressed the inhibitory responses of the lymphatic pump activity induced by PTHrP-(1-34) and S-nitroso- N-acetyl-penicillamine. The PTHrP-(1-34)-mediated inhibitory responses were significantly reduced by treatment with PTHrP-(7-34) (1 \u00d7 10<jats:sup>\u22127<\/jats:sup>M). These results suggest that PTHrP-(1-34) inhibits spontaneous pump activity of the isolated lymph vessels via PTH receptors and that production and release of endogenous nitric oxide and activation of ATP-sensitive K<jats:sup>+<\/jats:sup>channels in the lymph vessels contribute to the PTHrP-(1-34)-mediated inhibitory responses of the lymphatic pump activity.<\/jats:p>","DOI":"10.1152\/ajpheart.2001.281.1.h60","type":"journal-article","created":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T19:34:33Z","timestamp":1513971273000},"page":"H60-H66","source":"Crossref","is-referenced-by-count":19,"title":["Parathyroid hormone-related protein-(1-34) inhibits intrinsic pump activity of isolated murine lymph vessels"],"prefix":"10.1152","volume":"281","author":[{"given":"Risuke","family":"Mizuno","sequence":"first","affiliation":[{"name":"First Department of Physiology, Shinshu University School of Medicine, Matsumoto;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuyuki","family":"Ono","sequence":"additional","affiliation":[{"name":"Department of Electronics and Control Engineering, Nagano National College of Technology, Nagano, 381-8550; 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