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To gain molecular insights into HLHS, expression profiling by gene-chip microarray (Affymetrix U133 2.0) and by real-time RT-PCR was performed in the atrial septum of patients diagnosed with HLHS and compared with age-matched non-HLHS patients. Hierarchical clustering of all expressed genes with a P &lt; 0.01 of all tissue samples showed two main clusters, one of HLHS and the other of non-HLHS, suggesting different expression patterns by the two groups. Net affix followed by real-time RT-PCR analysis identified the differentially expressed genes to be those involved in chromatin remodeling, cell cycle regulation, and transcriptional regulation. These included remodeling factors, histone deactylase 2 and SET and MYND domain containing 1; transcription factors, FoxP1, and components of the calcineurin-nuclear factor of activated T cells signaling pathway; and cell cycle regulators, cyclin-dependent kinase (CDK)-4, phosphatase and tensin homolog, and p18. Since these factors play essential roles in heart growth and development, the abnormal expression pattern suggests that these molecules may contribute to the pathogenesis of HLHS.<\/jats:p>","DOI":"10.1152\/ajpheart.91494.2007","type":"journal-article","created":{"date-parts":[[2008,3,15]],"date-time":"2008-03-15T01:09:10Z","timestamp":1205543350000},"page":"H2268-H2275","source":"Crossref","is-referenced-by-count":17,"title":["Transcription repression and blocks in cell cycle progression in hypoplastic left heart syndrome"],"prefix":"10.1152","volume":"294","author":[{"given":"Katheryn","family":"Gambetta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamad K.","family":"Al-Ahdab","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel N.","family":"Ilbawi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nahidh","family":"Hassaniya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madhu","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","unstructured":"Andr\u00e9s V, Chiara MD, Mahdavi V.A new bipartite DNA-binding domain: cooperative interaction between the cut repeat and homeo domain of the cut homeo proteins.Genes Dev2: 245\u2013257, 1994."},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000197782.21444.8f"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.2174\/138920306776359740"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/S1054-8807(02)00127-8"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(03)00529-7"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1002\/bies.10212"},{"key":"R7","unstructured":"Cook AC.The phenotype during human fetal development. 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