{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:10:38Z","timestamp":1789985438993,"version":"4.0.1"},"reference-count":51,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:p>Cystic fibrosis (CF) is associated with severe neutrophilic airway inflammation. We showed that moxifloxacin (MXF) inhibits IL-8 and MAPK activation in monocytic and respiratory epithelial cells. Azithromycin (AZM) and ciprofloxacin (CIP) are used clinically in CF. Thus we now examined effects of MXF, CIP, and AZM directly on CF cells. IB3, a CF bronchial cell line, and corrected C38 cells were treated with TNF-\u03b1, IL-1\u03b2, or LPS with or without 5\u201350 \u03bcg\/ml MXF, CIP, or AZM. IL-6 and IL-8 secretion (ELISA), MAPKs ERK1\/2, JNK, p38, and p65 NF-\u03baB (Western blot) activation were measured. Baseline IL-6 was sixfold higher in IB3 than C38 cells but IL-8 was similar. TNF-\u03b1 and IL-1\u03b2 increased IL-6 and IL-8 12- to 67-fold with higher levels in IB3 than C38 cells post-TNF-\u03b1 ( P &lt; 0.05). Levels were unchanged following LPS. Baseline phosphorylated form of ERK1\/2 (p-ERK1\/2), JNK, and NF-\u03baB p65 were higher in IB3 than C38 cells (5-, 1.4-, and 1.4-fold), and following TNF-\u03b1 increased, as did the p-p38, by 1.6- to 2-fold. MXF (5\u201350 \u03bcg\/ml) and CIP (50 \u03bcg\/ml), but not AZM, suppressed IL-6 and IL-8 secretion by up to 69%. MXF inhibited TNF-\u03b1-stimulated MAPKs ERK1\/2, 46-kDa JNK, and NF-\u03baB up to 60%, 40%, and 40%, respectively. In contrast, MXF did not inhibit p38 activation, implying a highly selective pretranslational effect. In conclusion, TNF-\u03b1 and IL-1\u03b2 induce an exaggerated inflammatory response in CF airway cells, inhibited by MXF more than by CIP or AZM. Clinical trials are recommended to assess efficacy in CF and other chronic lung diseases.<\/jats:p>","DOI":"10.1152\/ajplung.00030.2006","type":"journal-article","created":{"date-parts":[[2006,9,30]],"date-time":"2006-09-30T00:56:20Z","timestamp":1159577780000},"page":"L343-L352","source":"Crossref","is-referenced-by-count":58,"title":["Moxifloxacin but not ciprofloxacin or azithromycin selectively inhibits IL-8, IL-6, ERK1\/2, JNK, and NF-\u03baB activation in a cystic fibrosis epithelial cell line"],"prefix":"10.1152","volume":"292","author":[{"given":"Hannah","family":"Blau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keren","family":"Klein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Itamar","family":"Shalit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Drora","family":"Halperin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ina","family":"Fabian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.22.1.3400"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200206-627OC"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh519"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200207-765OC"},{"key":"R5","unstructured":"Blau H, Fabian I, Kletter Y, Horev L, Kariv N, Shechtman I, Alteraz H, Ben-Neriah Y, Shalit I.Protective immunomodulation by moxifloxacin againstCandida albicanslung infection in cyclophosphamide treated mice: suppression of TNF-a, interleukin 8 and NF\u03baB activation. 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