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As we age, many components of pulmonary innate immunity are impaired, including slowing of mucociliary clearance. Ciliary beat frequency (CBF) is a major determinant of mucociliary clearance, and it slows as we age. We hypothesized that CBF is slowed in aging because of increased oxidative stress, which activates PKC\u03b5 signaling. We pharmacologically inhibited PKC\u03b5 in ex vivo mouse models of aging. We measured a slowing of CBF with aging that was reversed with inhibition using the novel PKC inhibitor, Ro-31-8220, as well as the PKC\u03b5 inhibitor, PKCe141. Inhibition of PKC\u03b5 using siRNA in mouse trachea also returned CBF to normal. In addition, antioxidants decrease PKC\u03b5 activity and speed cilia. We also aged wild-type and PKC\u03b5 KO mice and measured CBF. The PKC\u03b5 KO mice were spared from the CBF slowing of aging. Using human airway epithelial cells from younger and older donors at air-liquid interface (ALI), we inhibited PKC\u03b5 with siRNA. We measured a slowing of CBF with aging that was reversed with siRNA inhibition of PKC\u03b5. In addition, we measured bead clearance speeds in human ALI, which demonstrated a decrease in bead velocity with aging and a return to baseline after inhibition of PKC\u03b5. In summary, in human and mouse models, aging is associated with increased oxidant stress, which activates PKC\u03b5 and slows CBF. <\/jats:p>","DOI":"10.1152\/ajplung.00033.2018","type":"journal-article","created":{"date-parts":[[2018,9,13]],"date-time":"2018-09-13T09:37:10Z","timestamp":1536831430000},"page":"L882-L890","source":"Crossref","is-referenced-by-count":37,"title":["Oxidative stress associated with aging activates protein kinase C\u03b5, leading to cilia slowing"],"prefix":"10.1152","volume":"315","author":[{"given":"Kristina L.","family":"Bailey","sequence":"first","affiliation":[{"name":"Pulmonary, Critical Care, Sleep and Allergy Division, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska"},{"name":"Veterans Affairs Nebraska-Western Iowa Health Care System, Omaha, Nebraska"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kusum K.","family":"Kharbanda","sequence":"additional","affiliation":[{"name":"Pulmonary, Critical 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