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However, there is limited information about the effects of VILI on the intracellular form of surfactant, the lamellar body. It is hypothesized that VILI leads to alterations of lamellar body numbers and function. To test this hypothesis, rats were randomized to one of three groups, nonventilated controls, control ventilation, and high tidal volume ventilation (VILI). Following physiological assessment to confirm lung injury, isolated lamellar bodies were tested for surfactant function on a constrained sessile drop surfactometer. A separate cohort of animals was used to fix the lungs followed by examination of lamellar body numbers and morphology using transmission electron microscopy. The results showed an impaired ability of reducing surface tension for the lamellar bodies isolated from the VILI group as compared with the two other groups. The morphological assessment revealed that the number, and the relative area covered by, lamellar bodies were significantly decreased in animals with VILI animals as compared with the other groups. It is concluded that VILI causes significant alterations to lamellar bodies. It is speculated that increased secretion causes a depletion of lamellar bodies that cannot be compensated by de novo synthesis of surfactant in these injured lungs.<\/jats:p>","DOI":"10.1152\/ajplung.00055.2017","type":"journal-article","created":{"date-parts":[[2017,5,25]],"date-time":"2017-05-25T20:10:20Z","timestamp":1495743020000},"page":"L524-L533","source":"Crossref","is-referenced-by-count":22,"title":["Impact of ventilation-induced lung injury on the structure and function of lamellar bodies"],"prefix":"10.1152","volume":"313","author":[{"given":"Scott","family":"Milos","sequence":"first","affiliation":[{"name":"Lawson Health Research Institute, Western University, London Ontario, Canada;"},{"name":"Department of Physiology and Pharmacology, Western University, London Ontario, Canada;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Khazaee","sequence":"additional","affiliation":[{"name":"Lawson Health Research Institute, Western University, 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