{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T15:31:43Z","timestamp":1770910303901,"version":"3.50.1"},"reference-count":38,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:p>Pulmonary neuroepithelial bodies (NEB) form innervated cell clusters that express voltage-activated currents and function as airway O<jats:sub>2<\/jats:sub>sensors. We investigated A-type K<jats:sup>+<\/jats:sup>currents in NEB cells using neonatal rabbit lung slice preparation. The whole cell K<jats:sup>+<\/jats:sup>current was slowly inactivating with activation threshold of \u223c\u221230 mV. This current was blocked \u223c27% by blood-depressing substance I (BDS-I; 3 \u03bcM), a selective blocker of Kv3.4 subunit, and reduced \u223c20% by tetraethylammonium (TEA; 100 \u03bcM). The BDS-I-sensitive component had an average peak value of 189 \u00b1 14 pA and showed fast inactivation kinetics that could be fitted by one-component exponential function with a time constant of (\u03c41) 77 \u00b1 10 ms. This Kv slowly inactivating current was also blocked by heteropodatoxin-2 (HpTx-2; 0.2 \u03bcM), a blocker of Kv4 subunit. The HpTx-2-sensitive current had an average peak value of 234 \u00b1 23 pA with a time constant (\u03c4) 82 \u00b1 11 ms. Hypoxia (Po<jats:sub>2<\/jats:sub>= 15\u201320 mmHg) inhibited the slowly inactivating K<jats:sup>+<\/jats:sup>current by \u223c47%, during voltage steps from \u221230 to +30 mV, and no further inhibition occurred when TEA was combined with hypoxia. Nicotine at concentrations of 50 and 100 \u03bcM suppressed the slowly inactivating K<jats:sup>+<\/jats:sup>current by \u223c24 and \u223c40%, respectively. This suppression was not reversed by mecamylamine suggesting a direct effect of nicotine on these K<jats:sup>+<\/jats:sup>channels. In situ hybridization experiments detected expression of mRNAs for Kv3.4 and Kv4.3 subunits, while double-label immunofluorescence confirmed membrane localization of respective channel proteins in NEB cells. These studies suggest that the hypoxia-sensitive current in NEB cells is carried by slowly inactivating A-type K<jats:sup>+<\/jats:sup>channels, which underlie their oxygen-sensitive potassium currents, and that exposure to nicotine may directly affect their function, contributing to smoking-related lung disease.<\/jats:p>","DOI":"10.1152\/ajplung.00098.2007","type":"journal-article","created":{"date-parts":[[2007,7,21]],"date-time":"2007-07-21T01:09:16Z","timestamp":1184980156000},"page":"L892-L902","source":"Crossref","is-referenced-by-count":21,"title":["Characterization of slowly inactivating KV\u03b1 current in rabbit pulmonary neuroepithelial bodies: effects of hypoxia and nicotine"],"prefix":"10.1152","volume":"293","author":[{"given":"Xiao 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