{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:28:52Z","timestamp":1787167732415,"version":"build-2736575974"},"reference-count":48,"publisher":"American Physiological Society","issue":"3","funder":[{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL111497"],"award-info":[{"award-number":["HL111497"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL142906"],"award-info":[{"award-number":["HL142906"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012803","name":"Rosalind Franklin University of Medicine and Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012803","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2023,3,1]]},"abstract":"<jats:p>\n                    Severe levels of acidosis (pH &lt; 6.8) have been shown to cause a sustained rise in cytosolic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentration in carotid body Type 1 (glomus) cells. To understand how physiologically relevant levels of acidosis regulate Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signaling in glomus cells, we studied the effects of small changes in extracellular pH (pH\n                    <jats:sub>o<\/jats:sub>\n                    ) on the kinetics of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    oscillations. A decrease in pH\n                    <jats:sub>o<\/jats:sub>\n                    from 7.4 to 7.3 (designated mild) and 7.2 (designated moderate) acidosis produced significant increases in the frequency and amplitude of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    oscillations. These effects of acidosis on Ca\n                    <jats:sup>2+<\/jats:sup>\n                    oscillations were not blocked by NS383 and amiloride [acid-sensing ion channel (ASIC) inhibitors]. Mild and moderate levels of acidosis, however, caused a small but significant inhibition of two-pore domain acid-sensing K\n                    <jats:sup>+<\/jats:sup>\n                    channels (TASK) (TASK-1- and TASK-3-like channels) and depolarized the cell by 6\u201313 mV. Acidosis-induced increase in Ca\n                    <jats:sup>2+<\/jats:sup>\n                    oscillations was inhibited by nifedipine (1 \u00b5M; L-type Ca\n                    <jats:sub>v<\/jats:sub>\n                    inhibitor) and by TTA-P2 (20 \u00b5M; T-type Ca\n                    <jats:sub>v<\/jats:sub>\n                    inhibitor). Mild inhibition of TASK activity by N-[(2,4-difluorophenyl)methyl]-2\u2032-[[[2-(4methoxyphenyl)acetyl]amino]methyl][1,1\u2032-biphenyl]-2-carboxamide (A1899) (0.3 \u00b5M) and 1-[1-[6-[[1,1\u2032-biphenyl]-4-ylcarbonyl)-5,6,7,8-tetrahydropyrido[4,3- d]pyrimidine-4-yl]-4-piperidinyl]-1-butanon (PK-THPP) (0.1 \u00b5M) increased Ca\n                    <jats:sup>2+<\/jats:sup>\n                    oscillation frequency to levels similar to those observed with mild-moderate acidosis. Mild acidosis (pH\n                    <jats:sub>o<\/jats:sub>\n                    7.3) and mild hypoxia (\u223c5%O\n                    <jats:sub>2<\/jats:sub>\n                    ) produced similar levels of changes in the kinetics of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    oscillations. Block of tetraethylammonium (TEA)-sensitive K\n                    <jats:sub>v<\/jats:sub>\n                    channels did not affect acid-induced increase in Ca\n                    <jats:sup>2+<\/jats:sup>\n                    oscillations. Our study shows that mild and moderate levels of acidosis increase the frequency and amplitude of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    oscillations primarily by inhibition of TASK without involving ASICs, and suggests a major role of TASK for signal transduction in response to a physiological change in pH\n                    <jats:sub>o<\/jats:sub>\n                    .\n                  <\/jats:p>","DOI":"10.1152\/ajplung.00099.2022","type":"journal-article","created":{"date-parts":[[2023,1,24]],"date-time":"2023-01-24T08:00:22Z","timestamp":1674547222000},"page":"L259-L270","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["TASK inhibition by mild acidosis increases Ca\n                    <sup>2+<\/sup>\n                    oscillations to mediate pH sensing in rat carotid body chemoreceptor cells"],"prefix":"10.1152","volume":"324","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2461-1207","authenticated-orcid":false,"given":"Donghee","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James O.","family":"Hogan","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, Illinois, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0535-6968","authenticated-orcid":false,"given":"Carl","family":"White","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Chicago Medical School, Rosalind Franklin University of Medicine and Science, 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