{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T04:32:55Z","timestamp":1790483575657,"version":"4.1.0"},"reference-count":45,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2002,11,1]]},"abstract":"<jats:p>Many studies indicate that hypoxic inhibition of some K<jats:sup>+<\/jats:sup>channels in the membrane of the pulmonary arterial smooth muscle cells (PASMCs) plays a part in initiating hypoxic pulmonary vasoconstriction. The sensitivity of the K<jats:sup>+<\/jats:sup>current ( I<jats:sub>k<\/jats:sub>), resting membrane potential ( E<jats:sub>m<\/jats:sub>), and intracellular Ca<jats:sup>2+<\/jats:sup>concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) of PASMCs to different levels of hypoxia in these cells has not been explored fully. Reducing Po<jats:sub>2<\/jats:sub>levels gradually inhibited steady-state I<jats:sub>k<\/jats:sub>of rat resistance PASMCs and depolarized the cell membrane. The block of I<jats:sub>k<\/jats:sub>by hypoxia was voltage dependent in that low O<jats:sub>2<\/jats:sub>tensions (3 and 0% O<jats:sub>2<\/jats:sub>) inhibited I<jats:sub>k<\/jats:sub>more at 0 and \u221220 mV than at 50 mV. As expected, the hypoxia-sensitive I<jats:sub>k<\/jats:sub>was also 4-aminopyridine sensitive. Fura 2-loaded PASMCs showed a graded increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>as Po<jats:sub>2<\/jats:sub>levels declined. This increase was reduced markedly by nifedipine and removal of extracellular Ca<jats:sup>2+<\/jats:sup>. We conclude that, as in the carotid body type I cells, PC-12 pheochromocytoma cells, and cortical neurons, increasing severity of hypoxia causes a proportional decrease in I<jats:sub>k<\/jats:sub>and E<jats:sub>m<\/jats:sub>and an increase of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>.<\/jats:p>","DOI":"10.1152\/ajplung.00104.2002","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:19:27Z","timestamp":1425413967000},"page":"L1143-L1150","source":"Crossref","is-referenced-by-count":48,"title":["Graded response of K<sup>+<\/sup>current, membrane potential, and [Ca<sup>2+<\/sup>]<sub>i<\/sub>to hypoxia in pulmonary arterial smooth muscle"],"prefix":"10.1152","volume":"283","author":[{"given":"Andrea","family":"Olschewski","sequence":"first","affiliation":[{"name":"Justus Liebig University, 35392 Giessen, Germany; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigang","family":"Hong","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center, University of Minnesota, Minneapolis, Minnesota 55417"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel P.","family":"Nelson","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center, University of Minnesota, Minneapolis, Minnesota 55417"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. 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