{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:05:54Z","timestamp":1791295554974,"version":"4.1.0"},"reference-count":33,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2002,2,1]]},"abstract":"<jats:p>We tested the novel hypothesis that neutrophils in the lung or the airspaces may produce hepatocyte growth factor (HGF) in ventilated patients with acute respiratory failure. Neutrophils were purified from blood and bronchoalveolar lavage (BAL) fluid samples from 16 mechanically ventilated patients who underwent BAL for a diagnostic workup of ventilator-acquired pneumonia. Most of the patients had pneumonia ( n = 11). Ten nonventilated patients served as controls. Both blood and BAL neutrophils released HGF in vitro. Basal HGF secretion by blood neutrophils from controls was 823 (666) pg \u00b7 ml<jats:sup>\u22121<\/jats:sup>\u00b7 10<jats:sup>\u22127<\/jats:sup>neutrophils (median, 25th\u201375th percentile) and doubled to 1,730 (1,684\u20132,316) pg \u00b7 ml<jats:sup>\u22121<\/jats:sup>\u00b7 10<jats:sup>\u22127<\/jats:sup>neutrophils ( P = 0.001) with lipopolysaccharide (LPS) stimulation. Basal HGF secretion by blood neutrophils from patients was similar [956 (655\u20132,140) pg \u00b7 ml<jats:sup>\u22121<\/jats:sup>\u00b7 10<jats:sup>\u22127<\/jats:sup>neutrophils, P = 0.4] and doubled with LPS stimulation [2,767 (2,165\u20133,688) pg \u00b7 ml<jats:sup>\u22121<\/jats:sup>\u00b7 10<jats:sup>\u22127<\/jats:sup>neutrophils, P &lt; 0.0001 vs. controls]. Alveolar neutrophils released HGF in vitro [653 (397\u20131,209) pg \u00b7 ml<jats:sup>\u22121<\/jats:sup>\u00b7 10<jats:sup>\u22127<\/jats:sup>neutrophils]. LPS stimulation did not significantly increase the HGF release from alveolar neutrophils [762 (434\u20131,305) pg \u00b7 ml<jats:sup>\u22121<\/jats:sup>\u00b7 10<jats:sup>\u22127<\/jats:sup>neutrophils]. BAL HGF positively correlated with the BAL neutrophil count ( P = 0.01, R = 0.58). We conclude that blood and alveolar neutrophils from patients with acute respiratory failure can produce HGF, a mitogenic factor that may enhance the alveolar repair process.<\/jats:p>","DOI":"10.1152\/ajplung.00121.2001","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:15:53Z","timestamp":1425413753000},"page":"L310-L315","source":"Crossref","is-referenced-by-count":27,"title":["Hepatocyte growth factor is produced by blood and alveolar neutrophils in acute respiratory failure"],"prefix":"10.1152","volume":"282","author":[{"given":"Sandrine","family":"Jaffr\u00e9","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale Unit\u00e9 408 and 479, Facult\u00e9 Xavier Bichat, D\u00e9partement d'Anesth\u00e9sie-R\u00e9animation, Laboratoire de Biochimie A et Service de Pneumologie, H\u00f4pital Bichat, Assistance Publique-H\u00f4pitaux de Paris, 75877 Paris, 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