{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:37:15Z","timestamp":1790649435813,"version":"4.1.0"},"reference-count":47,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2017,10,1]]},"abstract":"<jats:p> Macrolides antibiotics have been effectively used in many chronic diseases, especially with Pseudomonas aeruginosa (P. aeruginosa) infection. The mechanisms underlying the therapeutic effects of macrolides in these diseases remain poorly understood. We established a mouse model of chronic lung infection using P. aeruginosa agar-beads, with azithromycin treatment or placebo. Lung injury, bacterial clearance, and inflammasome-related proteins were measured. In vitro, the inflammasomes activation induced by flagellin or ATP were assessed in LPS-primed macrophages with or without macrolides treatment. Plasma IL-18 levels were determined from patients who were diagnosed with bronchiectasis isolated with or without P. aeruginosa and treated with azithromycin for 3\u20135 days. Azithromycin treatment enhanced bacterial clearance and attenuated lung injury in mice chronically infected with P. aeruginosa, which resulted from the inhibition of caspase-1-dependent IL-1\u03b2 and IL-18 secretion. In vitro, azithromycin and erythromycin inhibited NLRC4 and NLRP3 inflammasomes activation. Plasma IL-18 levels were higher in bronchiectasis patients with P. aeruginosa isolation compared with healthy controls. Azithromycin administration markedly decreased IL-18 secretion in bronchiectasis patients. The results of this study reveal that azithromycin and erythromycin exert a novel anti-inflammatory effect by attenuating inflammasomes activation, which suggests potential treatment options for inflammasome-related diseases. <\/jats:p>","DOI":"10.1152\/ajplung.00123.2017","type":"journal-article","created":{"date-parts":[[2017,7,6]],"date-time":"2017-07-06T20:40:30Z","timestamp":1499373630000},"page":"L677-L686","source":"Crossref","is-referenced-by-count":28,"title":["Macrolides protect against <i>Pseudomonas aeruginosa<\/i> infection via inhibition of inflammasomes"],"prefix":"10.1152","volume":"313","author":[{"given":"Li-Chao","family":"Fan","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie-Lu","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Wei","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-Wen","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao-Xue","family":"Ge","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Infectious Diseases, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China;"},{"name":"Department of Microbiology and Immunology, Tongji University School of Medicine, Shanghai, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augustine M. 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