{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T20:25:01Z","timestamp":1785270301216,"version":"3.55.0"},"reference-count":42,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2003,3,1]]},"abstract":"<jats:p>Decorin, a small leucin-rich proteoglycan, is a negative regulator of transforming growth factor-\u03b2, but the antifibrotic effect of decorin gene transfer has not been examined in a mouse model of usual interstitial pneumonia (UIP). We constructed a replication-defective recombinant adenovirus harboring human decorin gene (AdCMV.DC) and administered 1 \u00d7 l0<jats:sup>9<\/jats:sup>plaque-forming units of AdCMV.DC intratracheally or intravenously to C57BL\/6 mice with intraperitoneal injection of bleomycin, which induces a subpleural fibroproliferation, mimicking UIP, by day 28. Only intratracheal administration of AdCMV.DC increased decorin mRNA expression in the lung and decreased the hydroxyproline content augmented in bleomycin-induced pulmonary fibrosis (1.13 \u00b1 0.02 to 0.96 \u00b1 0.02, P = 0.006). In contrast, intravenous administration of AdCMV.DC increased the decorin expression only in the liver, but not in the lung, and without reducing lung fibrosis. These results indicate that adenoviral decorin gene transfer is effective only by direct administration to fibrosing lungs.<\/jats:p>","DOI":"10.1152\/ajplung.00131.2002","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:20:33Z","timestamp":1425414033000},"page":"L526-L532","source":"Crossref","is-referenced-by-count":35,"title":["Intratracheal gene transfer of decorin reduces subpleural fibroproliferation induced by bleomycin"],"prefix":"10.1152","volume":"284","author":[{"given":"Minoru","family":"Shimizukawa","sequence":"first","affiliation":[{"name":"Department of Respiratory Oncology and Molecular Medicine, Institute of Development, Aging, and Cancer, Tohoku University, Sendai 980-8575; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahito","family":"Ebina","sequence":"additional","affiliation":[{"name":"Department of Respiratory Oncology and Molecular Medicine, Institute of Development, Aging, and Cancer, Tohoku 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