{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:26:58Z","timestamp":1791149218143,"version":"4.1.0"},"reference-count":48,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2013,2,1]]},"abstract":"<jats:p> This study was designed to assess the relative importance of Cl<jats:sup>\u2212<\/jats:sup> and HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> secretion to mucociliary transport rate (MCT) in ex vivo porcine tracheas. MCT was measured in one group of tissues that was exposed to adventitial HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>-free solution while a parallel group was exposed to adventitial HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>-replete solution. After measurement of baseline MCT rates, acetylcholine (ACh) was added to stimulate submucosal gland mucous liquid secretion, and MCT rates were again measured. Before ACh addition, the mean MCT was higher in the HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>-free group (4.2 \u00b1 0.9 mm\/min) than in the HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>-replete group (2.3 \u00b1 0.3 mm\/min), but this difference was not statistically significant. ACh addition significantly increased MCT in both groups, but ACh-stimulated MCT was significantly lower in the HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>-free group (11.0 \u00b1 1.5 mm\/min) than in the HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>-replete group (17.0 \u00b1 2.0 mm\/min). A second series of experiments examined the effect on MCT of blocking Cl<jats:sup>\u2212<\/jats:sup> secretion with 100 \u03bcM bumetanide. Before adding ACh, MCT in the bumetanide-treated group (1.0 \u00b1 0.2 mm\/min) was significantly lower than in the control group (3.8 \u00b1 1.1 mm\/min). ACh addition significantly increased MCT in both groups, but there was no significant difference between the bumetanide-treated group (21.4 \u00b1 1.7 mm\/min) and control group (19.5 \u00b1 3.4 mm\/min). These results indicate that ACh-stimulated MCT has greater dependence on HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> secretion, whereas the basal MCT rate has greater dependence on Cl<jats:sup>\u2212<\/jats:sup> secretion. <\/jats:p>","DOI":"10.1152\/ajplung.00143.2012","type":"journal-article","created":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T20:34:33Z","timestamp":1354394073000},"page":"L184-L190","source":"Crossref","is-referenced-by-count":48,"title":["Mucociliary transport in porcine trachea: differential effects of inhibiting chloride and bicarbonate secretion"],"prefix":"10.1152","volume":"304","author":[{"given":"Jeffrey L.","family":"Cooper","sequence":"first","affiliation":[{"name":"Department of Physiology, College of Medicine, University of South Alabama, Mobile, Alabama;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul M.","family":"Quinton","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Rady Children's 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