{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T02:04:28Z","timestamp":1791079468978,"version":"4.1.0"},"reference-count":52,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:p>Pseudomonas aeruginosa is a leading cause of hospital-acquired pneumonia and an important pathogen in patients with chronic lung disease, such as cystic fibrosis and bronchiectasis. The contribution of Toll-like receptor 5 (TLR5) to the innate immune response to this organism is incompletely understood. We exposed wild-type and TLR5-deficient ( Tlr5<jats:sup>\u2212\/\u2212<\/jats:sup>) mice to aerosolized P. aeruginosa at low and high inocula and assessed bacterial clearance, lung inflammation, and cytokine production 4 and 24 h after infection. Bacterial clearance was impaired in Tlr5<jats:sup>\u2212\/\u2212<\/jats:sup>mice after low-inoculum, but not high-inoculum, infection. Early bronchoalveolar accumulation of neutrophils was reduced in Tlr5<jats:sup>\u2212\/\u2212<\/jats:sup>mice after low- and high-dose infection. Cytokine responses, including markedly impaired monocyte chemoattractant protein-1 production 4 h after low- and high-inoculum challenge, were selectively altered in Tlr5<jats:sup>\u2212\/\u2212<\/jats:sup>mice. In contrast, there was no impairment of bacterial clearance, neutrophil recruitment, or monocyte chemoattractant protein-1 production in Tlr5<jats:sup>\u2212\/\u2212<\/jats:sup>mice after infection with a nonflagellated isotypic strain of P. aeruginosa . Thus TLR5-mediated recognition of flagellin is involved in activating pulmonary defenses against P. aeruginosa and contributes to antibacterial resistance in a manner that is partially inoculum dependent. These data are the first to demonstrate a unique role for TLR5 in the innate immune response to P. aeruginosa lung infection.<\/jats:p>","DOI":"10.1152\/ajplung.00155.2009","type":"journal-article","created":{"date-parts":[[2009,10,3]],"date-time":"2009-10-03T13:55:20Z","timestamp":1254578120000},"page":"L1112-L1119","source":"Crossref","is-referenced-by-count":66,"title":["Role of Toll-like receptor 5 in the innate immune response to acute P. aeruginosa pneumonia"],"prefix":"10.1152","volume":"297","author":[{"given":"Amy E.","family":"Morris","sequence":"first","affiliation":[{"name":"Harborview Medical Center and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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