{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T03:44:44Z","timestamp":1774323884166,"version":"3.50.1"},"reference-count":69,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2012,9,1]]},"abstract":"<jats:p> Hypoxia contracts the pulmonary vein, but the underlying cellular effectors remain unclear. Utilizing contractile studies and whole cell patch-clamp electrophysiology, we report for the first time a hypoxia-sensitive K<jats:sup>+<\/jats:sup> current in porcine pulmonary vein smooth muscle cells (PVSMC). Hypoxia induced a transient contractile response that was 56 \u00b1 7% of the control response (80 mM KCl). This contraction required extracellular Ca<jats:sup>2+<\/jats:sup> and was sensitive to Ca<jats:sup>2+<\/jats:sup> channel blockade. Blockade of K<jats:sup>+<\/jats:sup> channels by tetraethylammonium chloride (TEA) or 4-aminopyridine (4-AP) reversibly inhibited the hypoxia-mediated contraction. Single-isolated PVSMC (typically 159.1 \u00b1 2.3 \u03bcm long) had mean resting membrane potentials (RMP) of \u221236 \u00b1 4 mV with a mean membrane capacitance of 108 \u00b1 3.5 pF. Whole cell patch-clamp recordings identified a rapidly activating, partially inactivating K<jats:sup>+<\/jats:sup> current ( I<jats:sub>KH<\/jats:sub>) that was hypoxia, TEA, and 4-AP sensitive. I<jats:sub>KH<\/jats:sub> was insensitive to Penitrem A or glyburide in PVSMC and had a time to peak of 14.4 \u00b1 3.3 ms and recovered in 67 ms following inactivation at +80 mV. Peak window current was \u221232 mV, suggesting that I<jats:sub>KH<\/jats:sub> may contribute to PVSMC RMP. The molecular identity of the potassium channel is not clear. However, RT-PCR, using porcine pulmonary artery and vein samples, identified Kv<jats:sub>1.5<\/jats:sub>, Kv<jats:sub>2.1<\/jats:sub>, and BK, with all three being more abundant in the PV. Both artery and vein expressed STREX, a highly conserved and hypoxia-sensitive BK channel variant. Taken together, our data support the hypothesis that hypoxic inhibition of I<jats:sub>KH<\/jats:sub> would contribute to hypoxic-induced contraction in PVSMC. <\/jats:p>","DOI":"10.1152\/ajplung.00157.2012","type":"journal-article","created":{"date-parts":[[2012,7,7]],"date-time":"2012-07-07T00:44:41Z","timestamp":1341621881000},"page":"L476-L486","source":"Crossref","is-referenced-by-count":9,"title":["Hypoxia sensitivity of a voltage-gated potassium current in porcine intrapulmonary vein smooth muscle cells"],"prefix":"10.1152","volume":"303","author":[{"given":"Ciprian","family":"Dospinescu","sequence":"first","affiliation":[{"name":"School of Pharmacy and Life Sciences, Robert Gordon University, Schoolhill, Aberdeen;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9l\u00e8ne","family":"Widmer","sequence":"additional","affiliation":[{"name":"Department of Biological and Biomedical Science, Glasgow Caledonian University, Glasgow, 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