{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T17:12:09Z","timestamp":1788628329882,"version":"build-2803163510"},"reference-count":27,"publisher":"American Physiological Society","issue":"3","funder":[{"DOI":"10.13039\/100015153","name":"Air Liquide Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015153","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2021,9,1]]},"abstract":"<jats:p> Intrauterine growth restriction (IUGR) increases the risk of bronchopulmonary dysplasia (BPD), one of the major complications of prematurity. Antenatal low-protein diet (LPD) exposure in rats induces IUGR and mimics BPD-related alveolarization disorders. Peroxisome proliferator-activated receptor-\u03b3 (PPAR\u03b3) plays a key role in normal lung development and was found deregulated following LPD exposure. The objective of this article was to investigate the effects of nebulized curcumin, a natural PPAR\u03b3 agonist, to prevent IUGR-related abnormal lung development. We studied rat pups antenatally exposed to an LPD or control diet (CTL) and treated with nebulized curcumin (50\u2009mg\/kg) or vehicle from postnatal ( P) days 1 to 5. The primary readouts were lung morphometric analyses at P21. Immunohistochemistry ( P21) and microarrays ( P6 and P11) were compared within animals exposed to LPD versus controls, with and without curcumin treatment. Quantitative morphometric analyses revealed that LPD induced abnormal alveolarization as evidenced by a significant increase in mean linear intercept (MLI) observed in P21 LPD-exposed animals. Early curcumin treatment prevented this effect, and two-way ANOVA analysis demonstrated significant interaction between diet and curcumin both for MLI [ F<jats:sub>(1,39)<\/jats:sub> = 12.67, P = 0.001] and radial alveolar count at P21 [ F<jats:sub>(1,40)<\/jats:sub> = 6.065, P = 0.0182]. Immunohistochemistry for fatty acid binding protein 4 (FABP4), a major regulator of PPAR\u03b3 pathway, showed a decreased FABP4<jats:sup>+<\/jats:sup> alveolar cell density in LPD-exposed animals treated by curcumin. Transcriptomic analysis showed that early curcumin significantly prevented the activation of profibrotic pathways observed at P11 in LPD-exposed animals. Nebulized curcumin appears to be a promising strategy to prevent alveolarization disorders in IUGR rat pups, targeting pathways involved in lung development. <\/jats:p>","DOI":"10.1152\/ajplung.00195.2021","type":"journal-article","created":{"date-parts":[[2021,6,23]],"date-time":"2021-06-23T12:01:38Z","timestamp":1624449698000},"page":"L545-L552","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Nebulized curcumin protects neonatal lungs from antenatal insult in rats"],"prefix":"10.1152","volume":"321","author":[{"given":"Cyril","family":"Guillier","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9 Et de la Recherche M\u00e9dicale (INSERM) U1141, Paris, France"},{"name":"Assistance Publique\u2014H\u00f4pitaux de Paris, Service de M\u00e9decine et R\u00e9animation n\u00e9onatales de Port-Royal, Paris, France"},{"name":"Universit\u00e9 Paris Descartes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"Carri\u00e8re","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 Et de la Recherche M\u00e9dicale (INSERM) U1141, Paris, France"},{"name":"Assistance Publique\u2014H\u00f4pitaux de Paris, Service de M\u00e9decine et R\u00e9animation n\u00e9onatales de Port-Royal, Paris, France"},{"name":"Universit\u00e9 Paris Descartes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Pansiot","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 Et de la Recherche M\u00e9dicale (INSERM) U1141, Paris, France"},{"name":"Universit\u00e9 Paris Diderot, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arielle","family":"Maroni","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 Et de la Recherche M\u00e9dicale (INSERM) U1141, Paris, France"},{"name":"Universit\u00e9 Paris Descartes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9217-0717","authenticated-orcid":true,"given":"Elodie","family":"Billion","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 Et de la Recherche M\u00e9dicale (INSERM) U1141, Paris, France"},{"name":"Assistance Publique\u2014H\u00f4pitaux de Paris, Service de M\u00e9decine et R\u00e9animation n\u00e9onatales de Port-Royal, Paris, France"},{"name":"Universit\u00e9 Paris Descartes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maud","family":"Ringot","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 Et de la Recherche M\u00e9dicale (INSERM) U1141, Paris, France"},{"name":"Universit\u00e9 Paris Diderot, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Fran\u00e7ois","family":"Benoist","sequence":"additional","affiliation":[{"name":"Assistance Publique\u2014H\u00f4pitaux de Paris, Service de Biochimie-Hormonologie, H\u00f4pital Robert Debr\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Jacques","sequence":"additional","affiliation":[{"name":"Genom\u2019ic. 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