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Loss of cystic fibrosis transmembrane conductance regulator (CFTR) function is associated with MAPK hyperactivation and increased cytokines expression, such as interleukin-8 [chemoattractant chemokine (C-X-C motif) ligand 8 (CXCL8)]. Recently, new therapeutic strategies directly targeting the basic CFTR defect have been developed, and ORKAMBI (Vx-809\/Vx-770 combination) is the only Food and Drug Administration-approved treatment for CF patients homozygous for the F508del mutation. Here we aimed to determine the effect of the Vx-809\/Vx-770 combination on the induction of the inflammatory response by fully differentiated primary bronchial epithelial cell cultures from CF patients carrying F508del mutations, following exposure to P. aeruginosa exoproducts. Our data unveiled that CFTR functional rescue with Vx-809\/Vx-770 drastically reduces CXCL8 (as well as CXCL1 and CXCL2) transcripts and p38 MAPK phosphorylation in response to P. aeruginosa exposure through a CFTR-dependent mechanism. These results suggest that ORKAMBI has anti-inflammatory properties that could decrease lung inflammation and contribute to the observed beneficial impact of this treatment in CF patients. <\/jats:p>","DOI":"10.1152\/ajplung.00198.2017","type":"journal-article","created":{"date-parts":[[2018,1,8]],"date-time":"2018-01-08T13:42:04Z","timestamp":1515418924000},"page":"L635-L641","source":"Crossref","is-referenced-by-count":45,"title":["Vx-809\/Vx-770 treatment reduces inflammatory response to <i>Pseudomonas aeruginosa<\/i> in primary differentiated cystic fibrosis bronchial epithelial cells"],"prefix":"10.1152","volume":"314","author":[{"given":"Manon","family":"Ruffin","sequence":"first","affiliation":[{"name":"Centre de recherche du centre hospitalier de l'universit\u00e9 de Montr\u00e9al (CRCHUM), Montr\u00e9al, Qu\u00e9bec, Canada"},{"name":"D\u00e9partement de M\u00e9decine, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Roussel","sequence":"additional","affiliation":[{"name":"The Meakins-Christie Laboratories at the Research Institute of the McGill University Health Center, McGill University, Montr\u00e9al, Qu\u00e9bec, Canada"},{"name":"Department of Medicine, McGill University, Montr\u00e9al, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9milie","family":"Maill\u00e9","sequence":"additional","affiliation":[{"name":"Centre de recherche du centre hospitalier de l'universit\u00e9 de Montr\u00e9al (CRCHUM), Montr\u00e9al, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Rousseau","sequence":"additional","affiliation":[{"name":"The Meakins-Christie Laboratories at the Research Institute of the McGill University Health Center, McGill University, Montr\u00e9al, Qu\u00e9bec, Canada"},{"name":"Department of Medicine, McGill University, Montr\u00e9al, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Brochiero","sequence":"additional","affiliation":[{"name":"Centre de recherche du centre hospitalier de l'universit\u00e9 de Montr\u00e9al (CRCHUM), Montr\u00e9al, Qu\u00e9bec, Canada"},{"name":"D\u00e9partement de M\u00e9decine, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit059"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.098566"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2015.03.003"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3008680"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2011-200393"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1038\/358761a0"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201609-1954OC"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00470"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12726"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200409059"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201500166R"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0127742"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00076214"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1105787108"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2013.06.008"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3008889"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E12-06-0424"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1409547"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-6-26"}],"container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajplung.00198.2017","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,24]],"date-time":"2019-09-24T10:21:22Z","timestamp":1569320482000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajplung.00198.2017"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,1]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4,1]]}},"alternative-id":["10.1152\/ajplung.00198.2017"],"URL":"https:\/\/doi.org\/10.1152\/ajplung.00198.2017","relation":{},"ISSN":["1040-0605","1522-1504"],"issn-type":[{"value":"1040-0605","type":"print"},{"value":"1522-1504","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4,1]]}}}