{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T11:16:31Z","timestamp":1761218191121,"version":"3.37.3"},"reference-count":41,"publisher":"American Physiological Society","issue":"1","funder":[{"DOI":"10.13039\/100000071","name":"HHS | NIH | National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R01-HD072842"],"award-info":[{"award-number":["R01-HD072842"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Lung Cellular and Molecular Physiology"],"published-print":{"date-parts":[[2020,1,1]]},"abstract":"<jats:p> Mechanical ventilation from birth with normal tidal volumes (V<jats:sub>T<\/jats:sub>) causes lung injury and systemic responses in preterm sheep. The addition of budesonide to surfactant therapy decreases these injury markers. Budesonide and surfactant will decrease the injury from injurious V<jats:sub>T<\/jats:sub> ventilation in preterm sheep. Lambs at 126\u2009\u00b1\u20091 day gestational age were ventilated from birth with either: 1) Normal V<jats:sub>T<\/jats:sub> [surfactant 200 mg\/kg before ventilation, positive end expiratory pressure (PEEP) 5 cmH<jats:sub>2<\/jats:sub>O, V<jats:sub>T<\/jats:sub> 8 mL\/kg] or 2) Injury V<jats:sub>T<\/jats:sub> (high pressure, 100% oxygen, no PEEP) for 15 min, then further randomized to surfactant + saline or surfactant + 0.25 mg\/kg budesonide with Normal V<jats:sub>T<\/jats:sub> for 6 h. Lung function and lung, liver, and brain tissues were evaluated for indicators of injury. Injury V<jats:sub>T<\/jats:sub> + saline caused significant injury and systemic responses, and Injury V<jats:sub>T<\/jats:sub> + budesonide improved lung physiology. Budesonide decreased lung inflammation and decreased pro-inflammatory cytokine mRNA in the lung, liver, and brain to levels similar to Normal V<jats:sub>T<\/jats:sub> + saline. Budesonide was present in plasma within 15 min of treatment in both ventilation groups, and less than 5% of the budesonide remained in the lung at 6 h. mRNA sequencing of liver and periventricular white matter demonstrated multiple pathways altered by both Injury V<jats:sub>T<\/jats:sub> and budesonide and the combination exposure. In lambs receiving Injury V<jats:sub>T<\/jats:sub>, the addition of budesonide to surfactant improved lung physiology and decreased pro-inflammatory cytokine responses in the lung, liver, and brain to levels similar to lambs receiving Normal V<jats:sub>T<\/jats:sub>. <\/jats:p>","DOI":"10.1152\/ajplung.00203.2019","type":"journal-article","created":{"date-parts":[[2019,10,16]],"date-time":"2019-10-16T08:56:11Z","timestamp":1571216171000},"page":"L41-L48","source":"Crossref","is-referenced-by-count":23,"title":["Surfactant plus budesonide decreases lung and systemic responses to injurious ventilation in preterm sheep"],"prefix":"10.1152","volume":"318","author":[{"given":"Noah H.","family":"Hillman","sequence":"first","affiliation":[{"name":"Division of Neonatology, Cardinal Glennon Children\u2019s Hospital, Saint Louis University, St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. 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