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Alterations in the bioenergetic profile of macrophages may also be influenced by their location within the respiratory tract. This study sought to characterize the bioenergetic profile of macrophages sampled from different locations within the respiratory tract at baseline and in response to ex vivo xenobiotic challenge. Surface macrophages recovered from healthy volunteers by induced sputum and by bronchial and bronchoalveolar lavage were profiled using extracellular flux analyses. Oxygen consumption and extracellular acidification rates were measured at rest and after stimulation with lipopolysaccharide (LPS), phorbol 12-myristate 13-acetate (PMA), or 1,2-naphthoquinone (1,2-NQ). Oxygen consumption and extracellular acidification rates were highly correlated for all macrophage samples. Induced sputum macrophages had relatively higher oxygen consumption and extracellular acidification rates and were largely reliant on glycolysis. In contrast, bronchial fraction and bronchoalveolar macrophages depended more heavily on mitochondrial respiration. Bronchoalveolar macrophages showed elevated LPS-induced cytokine responses. Unlike their autologous peripheral blood monocytes, lung macrophages from any source did not display bioenergetic changes following LPS stimulation. The protein kinase C activator PMA did not affect mitochondrial respiration, whereas the air pollutant 1,2-NQ induced marked mitochondrial dysfunction in bronchoalveolar and bronchial fraction macrophages. The bioenergetic characteristics of macrophages from healthy individuals are dependent on their location within the respiratory tract. These findings establish a regional bioenergetic profile for macrophages from healthy human airways that serves as a reference for changes that occur in disease. <\/jats:p>","DOI":"10.1152\/ajplung.00208.2018","type":"journal-article","created":{"date-parts":[[2018,8,9]],"date-time":"2018-08-09T08:27:43Z","timestamp":1533803263000},"page":"L752-L764","source":"Crossref","is-referenced-by-count":42,"title":["Macrophages from the upper and lower human respiratory tract are metabolically distinct"],"prefix":"10.1152","volume":"315","author":[{"given":"Katelyn S.","family":"Lavrich","sequence":"first","affiliation":[{"name":"Curriculum in Toxicology, University of North Carolina Chapel Hill, Chapel Hill, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam M.","family":"Speen","sequence":"additional","affiliation":[{"name":"Curriculum in Toxicology, University of North Carolina Chapel Hill, Chapel Hill, North 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