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The objective of the current study was to characterize the effect of NO on surfactant protein gene expression in primary rat type II pneumocytes cultured on a substratum that promoted the maintenance of type II cell phenotype. Exposure to a NO donor, S-nitroso-N-acetylpenicillamine (SNAP), decreased surfactant protein (SP)-A, (SP)-B, and (SP)-C mRNA levels in type II pneumocytes in a time- and dose-dependent manner. The effect was mediated in part by an increase in endothelin-1 secretion and a decrease in the intracellular messenger, phosphorylated ERK1\/2 mitogen-activated protein kinases (MAPK). Exposing type II pneumocytes to endothelin-1 receptor antagonists PD-156707 or bosentan before exposure to SNAP partially prevented the decrease in surfactant protein gene expression. The results showed that NO mediated the decrease in surfactant protein gene expression at least in part through an increase in endothelin-1 secretion and a decrease in phosphorylated ERK1\/2 MAPKs. <\/jats:p>","DOI":"10.1152\/ajplung.00210.2004","type":"journal-article","created":{"date-parts":[[2005,1,8]],"date-time":"2005-01-08T01:38:17Z","timestamp":1105148297000},"page":"L950-L957","source":"Crossref","is-referenced-by-count":15,"title":["Nitric oxide decreases surfactant protein gene expression in primary cultures of type II pneumocytes"],"prefix":"10.1152","volume":"288","author":[{"given":"Jae W.","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert F.","family":"Gonzalez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl J.","family":"Chapin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin","family":"Busch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey R.","family":"Fineman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge A.","family":"Gutierrez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00617-7"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199807000-00034"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00227.2001"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1007\/s00408-002-0102-y"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-200001000-00018"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1998.275.5.H1643"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114477"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1007\/BF03014268"},{"key":"R9","unstructured":"Camps M, Nichols A, and Arkinstall S. 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